磁性纳米分子印迹聚合物的制备及其对生物体内PQQ-DA痕量产物的研究  

Preparation of Magnetic Nanomolecularly Imprinted Polymers and Their Application in Investigation of Trace PQQ-DA Products in Organisms

作　　者：毛师师[1] 周杏琴[1] 钦晓峰[1] 徐希杰[1] 谢敏浩[1] MAO Shi-shi;ZHOU Xing-qin;QIN Xiao-feng;XU Xi-jie;XIE Min-hao(Key Laboratory of Nuclear Medicine/Ministry of Health,Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine,Wuxi 214063,China)

机构地区：[1]江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室,江苏无锡214063

出　　处：《分析测试学报》2019年第4期482-487,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(81371590)

摘　　要：以磁性Fe_3O_4为内核,对磁性纳米粒子表面进行包裹和修饰,制备了对吡咯并喹啉醌-多巴胺(PQQ-DA)具有高识别性、高选择性的磁性纳米分子印迹材料,并结合超高效液相色谱-质谱(UPLC-MS)测定了生物体内PQQ-DA的含量。结果显示,PQQ-DA在0.02～0.1 mg/mL范围内呈良好的线性关系,相关系数(r^2)为0.996 6,检出限为0.2×10^(-11) mg/mL,加标回收率为88.7%～95.5%,相对标准偏差为2.6%～3.2%。该方法灵敏度高、重复性好,可用于生物体内PQQ-DA痕量产物的检测。A pyrroloquinoquinone (PQQ )-dopamine (DA )magnetic nanoparticle imprinted polymers were prepared by the combination of PQQ-DA and magnetic nano-Fe 3O 4 as the core wrapped with SiO 2,which possess high recognition and high selectivity toward PQQ-DA and have great advantages in the separation,purification and enrichment of low concentration compounds in complex matrix of brain tissue after molecular imprinting modification.The magnetic nanoparticles were applied in the determination of trace PQQ-DA in brain tissue samples by ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS ).Results showed that,under the optimized conditions,there was a good linear relationship for PQQ-DA in the range of 0.02-0.1 mg/mL with a correlation coefficient ( r ^2 )of 0.996 6,and the detection limit was 0.2×10^-11 mg/mL.The recoveries for brain tissue samples were in the range of 88.7 %-95.5 %,with relative standard deviations ( n =6 )of 2.6 %-3.2 %.The method could be used in the detection of trace PQQ-DA in vivo with high sensitivity and good repeatability.

关 键 词：磁性纳米分子印迹 吡咯并喹啉醌-多巴胺(PQQ-DA) 超高效液相色谱-质谱(UPLC-MS) 固相萃取 痕量产物 

分 类 号：O657.7[理学—分析化学]