鄂西清风藤的化学成分研究  

Study on Chemical Constituents of Sabia Campanulata Wall. subsp. ritchieae

作　　者：王莉云 孙庆文[1] 穆淑珍[2] 李江[2] 邓璐璐[2] WANG Li-yun;SUN Qing-wen;MU Shu-zhen;LI Jiang;DENGLu-lu(Guiyang University of Chinese Medicine, Guizhou Guiyang 550022;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guizhou Guiyang 550014, China)

机构地区：[1]贵阳中医学院,贵州贵阳550022 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014

出　　处：《广州化工》2019年第7期102-104,107,共4页GuangZhou Chemical Industry

基　　金：国家自然科学基金项目(81560707)

摘　　要：采用75%乙醇对鄂西清风藤进行提取,利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和重结晶等方法对提取物进行分离纯化,并通过理化性质与波谱数据并结合文献对化合物结构进行鉴定,且从该植物中首次分离出6个化合物:齐墩果烷-9(11),12-二烯3-酮(1)、β-谷甾醇(2)、邻苯二甲酸异丁酯(3)、茴芹苦素(4)、3-oxo-11α-methoxyolean-12-ene(5)、3-氧-■-齐墩果二烯(6)。同时采用MTT法检测了化合物对Con A肝损伤细胞存活率的影响,结果显示化合物3,5和6,具有一定的抗肝损伤活性。The branches of Sabia campanulata Wall. subsp. ritchieae were extracted with 75% ethanol, then separated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, recrystallization, and so on, based on bioassay- guided method, while their structures were identified by physical and chemical properties and spectral data combined with literature. Six compounds were isolated and identified from this plant for the first time: Oleanane-9 (11 ), 12-diene-3-one ( 1 ),β-sitosterol ( 2 ), butyl isobutylphthalate ( 3 ), Pimpinellin ( 4 ), 3-oxo-11α-methoxyolean-12-ene ( 5 ), 3-oxo-△ 11, 13 (18 )-oleanene ( 6 ), and MTT results showed that compounds 3 , 5 and 6 exerted certain liver protection activity.

关 键 词：鄂西清风藤 化学成分 ConA抗肝损伤活性 

分 类 号：R284.1.[医药卫生—中药学]