佐剂对两种旋毛虫丝氨酸蛋白酶抑制剂免疫影响的研究  

Effects of adjuvant on two kinds of Trichinella serine protease inhibit

作　　者：刘照琨 关靖喆 路丽群 于鹏成 刘明旭 吴丽佳 路义鑫[1] LIU Zhao-kun;GUAN Jing-zhe;LU Li-qun;YU Peng-cheng;LIU Ming-xu;WU Li-jia;LU Yi-xin(Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development,College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学动物医学学院动物疾病防控技术与制剂创制重点实验室,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2019年第1期11-14,19,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金资助项目(31372427);黑龙江省自然科学基金资助项目(C2016030)

摘　　要：在寄生虫感染过程中,丝氨酸蛋白酶抑制剂在虫体与宿主相互作用间发挥着重要作用。在免疫过程中,不同佐剂的促免疫应答能力和提高免疫保护能力均不同。为了比较不同佐剂对两种丝氨酸蛋白酶抑制剂(TsAdSPI和TsKaSPI)免疫保护性的影响,通过对免疫剂量进行优化,应用间接ELISA的方法分析两种SPIs抗体应答,分别在肌肉虫攻击感染后第1天、第3天、第7天、第10天剖杀小鼠取成虫并计数,计算成虫减虫率。结果显示,用3种不同剂量(20、50μg和100μg)的重组蛋白免疫小鼠,50μg与100μg蛋白剂量免疫组诱导产生类似的抗体水平,且均高于20μg组;免疫组弗氏佐剂、ISA 206佐剂和铝佐剂分别与重组蛋白乳化后免疫,可诱导相似的免疫应答及成虫减虫率,其中佐剂ISA 206和氢氧化铝的保护效果更好。In the process of parasitic infection,serine protease inhibitors play an important role in the interaction between the insect body and the host.In the immunization process,the activities of different adjuvants and the ability to improve the immune protection are different.In order to compare the immune protective effects of the different adjuvants on the two serine protease inhibitors(TsAdSPI and TsKaSPI),we used ELISA to analyze the antibavy responses against the two spls at 1,3,7,and lodar after the muscular infection,and then,then mice were killed to calculate the adult worm reduction rate.The results showed that with the three different doses(20,50 and 100μg)of the recombinant protein immunized mice,the autibody production in 50μg and 100μg protein groups was similar,but was higher than 20μg group Recombinant proteins with Freund′s adjuvant,ISA 206 adjuvant or aluminum adjuvant induced similar immune response and adult worm reduction rate,and the protective effect of adjuvant ISA206 and aluminum hydroxide was better.

关 键 词：旋毛虫 丝氨酸蛋白酶抑制剂 剂量 佐剂 保护性 

分 类 号：R383.15[医药卫生—医学寄生虫学]