基于激光共聚焦扫描显微技术分析激光对扁藻的影响  

Analysis of Laser Irradiation Effect on Platymonas subcordiformis Using Laser Confocal Scanning Microscopy

作　　者：黄祥英[1] 方玉宏 HUANG Xiangying;FANG Yuhong(Chengyi University College, Jimei University, Xiamen 361020, Fujian, China;College of Physics and Information Engineering, Minnan Normal University, Zhangzhou 363099, Fujian, China;Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, Fujian, China)

机构地区：[1]集美大学诚毅学院,福建厦门361021 [2]闽南师范大学物理与信息工程学院,福建漳州363099 [3]福建师范大学光电与信息工程学院医学光电科学技术教育部重点实验室福建省光子技术重点实验室,福建福州350007

出　　处：《激光生物学报》2019年第2期131-135,共5页Acta Laser Biology Sinica

基　　金：集美大学诚毅学院青年科研基金项目(CK17010)

摘　　要：本文以亚心形扁藻为样品,波长1 341 nm的Nd∶YAP激光为光源,通过激光共聚焦扫描显微技术,研究Nd∶YAP激光辐照亚心形扁藻对亚心形扁藻叶绿体自体荧光强度和叶绿体面积大小的影响。Nd∶YAP激光辐照后的亚心形扁藻通过488 nm Ar^+激光激发获得亚心形扁藻自体荧光图像及其荧光光谱。结果表明,试验中除(10 W,60 s)辐照剂量组外,其余辐照剂量组均提高了亚心形扁藻的自体荧光强度,且所有的辐照剂量组均增大了亚心形扁藻的叶绿体面积。Nd∶YAP激光可刺激亚心形扁藻的叶绿体发育,促进藻细胞的生长,改善叶绿体光合作用的活性。Taking the Platymonas subcordiformis as sample and the Nd ∶ YAP laser (1 341 nm)as the light source, the effects on chloroplast autofluorescence intensity and chloroplast area of Platymonas subcordiformis irradiated by laser were studied using confocal laser scanning microscopy. The autofluorescence images and fluorescence spectra of the Platymonas subcordiformis irradiated by Nd ∶ YAP laser were obtained by the excitation of 488 nm Ar + laser. The results showed that all the laser irradiation dose, except the irradiation group with parameters of 10 W power and 60 s duration time, improved the autofluorescence intensity of Platymonas subcordiformis , and all the doses increased the chloroplast area. The laser can stimulate the chloroplast evelopment of Platymonas subcordiformis , promoted the growth of algal cells and improve the activity of chloroplast photosynthesis.

关 键 词：荧光光谱 自体荧光强度 激光共聚焦扫描 亚心形扁藻 叶绿体面积 

分 类 号：Q631[生物学—生物物理学]