忍冬藤多糖的提取工艺优化及清除亚硝酸盐阻断亚硝胺合成的研究  

Optimization of the Extraction of the Polysaccharides from Caulis Lonicerae and Their Abilities of Scavenging Nitrite and Blocking Nitrosamine Synthesis

作　　者：李振 李萍[3] LI Zhen;LI Ping(College of Pharmacy,Linyi University,Linyi 276005,China;Shandong Lunan Chinese Medicinal Materials Resources Development Engineering Technology Research Center,Linyi 276005,China;Linyi People's Hospital,Linyi 276003,China)

机构地区：[1]临沂大学药学院,山东临沂276005 [2]山东省鲁南中药材资源开发工程技术研究中心,山东临沂276005 [3]临沂市人民医院,山东临沂276003

出　　处：《现代食品科技》2019年第4期130-139,共10页Modern Food Science and Technology

基　　金：山东省自然科学基金项目(ZR2015PH029);临沂大学博士科研启动基金项目(LYDX2016BS082)

摘　　要：本研究对忍冬藤多糖进行提取、分离和纯化,测定了其清除亚硝酸盐和阻断亚硝胺合成的作用。试验以忍冬藤多糖得率为指标,采用响应面法优化超声辅助提取多糖工艺,通过Sevag法脱蛋白、醇沉,真空干燥得到多糖,在模拟胃酸的条件下,探讨了忍冬藤多糖对清除亚硝酸盐阻断亚硝胺合成的影响。结果标明,忍冬藤多糖最佳提取条件为:液料比为30:1,提取时间为40min,超声功率为210 W,提取温度70℃,在此条件下,忍冬藤多糖得率理论值为8.53%,验证试验结果多糖得率为8.46%,在质量浓度为500μg/mL时,对亚硝酸盐的清除率为90.44%,对亚硝胺合成的阻断率为71.24%。因此,采用响应面法优化超声辅助提取忍冬藤多糖工艺预测性良好,忍冬藤多糖具有清除亚硝酸盐与阻断亚硝胺合成的作用,本研究为忍冬藤进一步利用和开发其衍生生物活性多糖产品提供了理论依据。In this research,the extraction,isolation and purification of the polysaccharides from Caulis Lonicerae were performed,and their functions to removing nitrite and block the synthesis of nitrosamine were determined.The ultrasound-assisted polysaccharide extraction was optimized using the yield of polysaccharides from Caulis Lonicerae as the index,and the response surface method.The extracted polysaccharides was deproteinized using the Sevag method,precipitated using alcohol,and vacuum dried,before the examinations on their abilities of scavenging nitrite and blocking nitrosamine synthesis under simulated gastric acid conditions.The results indicated the optimal extraction conditions:the ratio of liquid to material,30:1;extraction time,40 min;ultrasonic power,210 W;extraction temperature,70 ℃. Under the optimal conditions,the theoretical yield of polysaccharides was 8.53%,while the experimental value was 8.46%;at a concentration of 500 μg/mL,the polysaccharide extract had a nitrite-scavenging rate of 90.44% and a blocking rate of 71.24% for nitrosamine synthesis. Therefore,the response surface method,which was used to optimize the ultrasound-assisted extraction of polysaccharides from Caulis Lonicerae,provided good quality predictions.The obtained polysaccharides were capable of removing nitrite and blocking the synthesis of nitrosamines.This research provides a theoretical basis for further utilization of Caulis Lonicerae and development of derived bioactive polysaccharide products.

关 键 词：忍冬藤 多糖 提取工艺 响应面法 亚硝酸盐 亚硝胺 

分 类 号：R284.2[医药卫生—中药学]