基于铜纳米粒子/氧化锌/石墨烯修饰电极的电化学方法测定硫酸卡那霉素  

Electrochemical Determination of Kanamycin Sulfate Based on Copper Nanoparticle/Zinc Oxide/Graphene Modified Electrode

作　　者：梁彩云[1,2] 刘凤平 张翠忠 张贞发[1,2] 韦远玲 彭金云[1,2] LIANG Cai-Yun;LIU Feng-Ping;ZHANG Cui-Zhong;ZHANG Zhen-Fa;WEI Yuan-Ling;PENG Jin-Yun(Guangxi Normal University for Nationalities, Chongzuo 532200, China;Guangxi Colleges and Universities Key Laboratory Breeding Base of Chemistry ofGuangxi Southwest Plant Resources, Chongzuo 532200, China)

机构地区：[1]广西民族师范学院 [2]广西高校桂西南特色植物资源化学重点实验室培育基地,崇左532200

出　　处：《分析化学》2019年第5期739-747,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21465004);2017年广西中青年能力提升项目(No.2017KY0832);广西民族师范学院科研经费项目(No.2018QN037)资助~~

摘　　要：在氧化锌/石墨烯(ZnO/GO)修饰ITO电极表面电沉积铜纳米粒子(CuNPs),制备了一种新型的电化学传感器,用于检测硫酸卡那霉素(KANA)。采用扫描电镜对制备的纳米材料以及修饰电极表面进行表征。优化后的测定条件为:在0.15 mol/L PBS缓冲溶液(pH 6.5)中,电沉积扫描圈数为40圈时, KANA在铜纳米粒子/氧化锌/石墨烯(CuNPs/ZnO/GO/ITO)电极上的电化学响应最大。KANA在电极表面的反应机理为单电子转移过程,修饰电极有效表面积为0.482 cm^2,是裸电极的2.42倍。在0.99～30.6μmol/L范围内,响应电流与KANA浓度呈良好的线性关系,线性方程为I_(pc)=-5.183c-4.544×10^(-6),R^2=0.9975,检出限为:0.31μmol/L,加标回收率为97.8%～103.6%。此传感器具有良好的稳定性与重现性,可用于药物中KANA的检测。A novel electrochemical sensor was prepared by electrodeposition of copper nanoparticles (CuNPs) on a zinc oxide/graphene (ZnO/GO) modified ITO electrode and applied to the determination of kanamycin sulfate (KANA). The morphology of material and modified electrodes was characterized by scanning electron microscope. With optimization of measurement conditions systematically, the result showed that the electrochemical response of KANA on CuNPs/ZnO/GO/ITO electrode was the largest when the number of electrodeposition scanning circles were 40 in 0.15 mol/L PBS (pH=6.5) solution. The mechanism of electrode reaction of KANA involved one electron process, and the effective area (0.482 cm 2) of CuNPs/ZnO/GO/ITO electrode was 2.42 times as large as that of a bare ITO electrode. Moreover, the linear response range for determination of KANA was 0.99-30.6 μmol/L, the linear equation was I pc =-5.183 c - 4.544×10^-6 with the detection limit of 0.31 μmol/L, and the recoveries of standard addition experiments were 97.8%-103.6%. The sensor shows good stability and reproducibility, and can be used to detect kanamycin sulfate in the drug samples.

关 键 词：氧化锌/石墨烯 铜纳米粒子 修饰电极 硫酸卡那霉素 

分 类 号：O657.1[理学—分析化学]