恩拉霉素人工抗原的合成与鉴定  

Synthesis and identification of enramycin artificial antigen

作　　者：叶健强[1,2] 余华 康润敏[1,2] 严玉宝[3] 谢晶 胡娟[3] 廖党金[1,2] 周岷江 肖璐[1,2] 崔鹏博[3] 曹冶 叶勇刚[1,2] 于吉锋 李兴玉[1,2] 林毅[1,2] 潘梦 魏甬[1,2] 戴卓建 YE Jian-qiang;YU Hua;KANG Run-min;YAN Yu-bao;XIE Jing;HU Juan;LIAO Dang-jin;ZHOU Min-jiang;XIAO Lu;CUI Peng-bo;CAO Ye;YE Yong-gang;YU Ji-feng;LI Xing-yu;LIN Yi;PAN Meng;WEI Yong;DAI Zhuo-jian(Sichuan Animal Science Academy,Chengdu 610066,China;Key Laboratory of Animal Genetics and Breeding of Sichuan Province,Chengdu 610066,China;Sichuan Entry-Exit Inspection and Quarantine Bureau,Chengdu 610041,China)

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]动物遗传育种实验室四川省重点实验室,成都610066 [3]四川出入境检验检疫局,成都610041

出　　处：《湖北农业科学》2019年第7期92-95,156,共5页Hubei Agricultural Sciences

基　　金：国家质检总局科技计划项目"进出口动物产品恩拉霉素药残检测技术研究"(2008IK013)

摘　　要：建立酶联免疫方法以检测食品中恩拉霉素的残留,采用戊二醛法,偶联半抗原恩拉霉素(Er)和载体牛血清白蛋白(BSA)或卵清白蛋白(OVA),制备人工免疫抗原和人工包被抗原,分别为Er-BSA和Er-OVA。经紫外光谱鉴定,在紫外270～280 nm波长处得到Er-BSA和Er-OVA特征性偶联吸收峰。试验结果表明人工合成的2个人工抗原均偶联成功。人工免疫抗原和人工包被抗原的偶联比均达到26∶1,为进一步制备抗血清和酶联检测方法的建立奠定了基础。In order to establish an enzyme-linked immunosorbent assay(ELISA) for examing residual Enramycin(Er) in food.The antibody with high titer and specificity must be firstly prepared. So it was necessary for antibody production to conjugate the hapten(Er) with a carrier protein to synthesize immunogent(antigen). Enramycin was coupled with carrier protein bovine serum albumin(BSA) to obtain immunogent(Er-BSA) by glutaraldehyde(GA) method and with ovalnumin(OVA) to obtain coating antigen(Er-OVA) by GA respectively. The successful linkage of Er-BSA and Er-OVA were identified by UV spectrophotometry. The UV spectral characterization demonstrated that Er-BSA and Er-OVA were successfully synthesized at 270~280 nm. The coupling ratios between Er and BSA or OVA were determined to be 26∶1, respectively.

关 键 词：恩拉霉素 抗原合成 戊二醛法 

分 类 号：S859.796[农业科学—临床兽医学]