PHZ处理对P.bANKA感染小鼠前炎性细胞因子分泌的影响  

Effects of PHZ Treatment on the Secretion of Proinflammatory Cytokines in P.bANKA Infected Mice

作　　者：姚潍 郑小鹏 陈光[1] Yao Wei;Zheng Xiaopeng;Chen Guang(Jiamusi University, The School of Basic Medical Sciences, Jiamusi 154007, China)

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《广东化工》2019年第7期25-26,9,共3页Guangdong Chemical Industry

摘　　要：目的:探讨PHZ处理对P.bANKA感染小鼠前炎性细胞因子分泌的影响。方法:建立PHZ处理的P.bANKA感染的C57BL/6鼠疟模型,计数红细胞感染率;感染后第0、3和5天制备脾细胞悬液,ELISA检测脾脏中INF-γ和TNF-α分泌水平的变化。结果:与P.bANKA感染组小鼠相比,P.b+PHZ组小鼠虫血症增高明显,生存期明显缩短;于感染后第3天和5天TNF-α明显减少;于感染后3天IFN-γ水平也显著下降。结论:PHZ处理,明显的增加了P.bANKA感染早期小鼠原虫血症水平,生存期显著缩短。可能与贫血的P.y17XL感染早期C57BL/6小鼠分泌的TNF-α和IFN-γ水平降低,不能有效的杀死红内期疟原虫密切相关,确切机制还有待进一步研究。Intention: This article explores the effects of PHZ treatment on the secretion of proinflammatory cytokines in P.bANKA infected mice. Method: We established the malaria model of PHZ treatment in P.bANKA infected C57 BL/6 mice and measured the infection rate of erythrocyte;We prepared the suspension liquid of spleen cells on 0, 3 rd, 5 th day after infection and measured the changes of secretion level of TNF-α and IFN-γ by ELISA in spleen. Result: Compared with the mice infected with P.bANKA, the serum parasitemia was significantly increased and the survival durations were shorter in P.b+PHZ group mice;The level of TNF-α was significantly decreased on 3 rd and 5 th day;The level of IFN-γ was also decreased markedly on 3 th day. Conclusion: PHZ treatment significantly increased the level of parasitemia in P.bANKA early infected mice and the survival durations were shorter. It may be closely related to the derease of TNF-α and IFN-γ secreted anemic P.y17 XL early infected C57 BL/6 mice leads to the inability to effectively kill the plasmodium in erythrocyte stage. But the exact mechanism needs futher investigation.

关 键 词：PHZ P.bANKA 前炎性细胞因子 TNF-Α IFN-Γ 

分 类 号：TQ[化学工程]