小桐子bZIP转录因子的全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of bZIP Transcription Factor Family in Jatropha curcas

作　　者：王海波[1] 郭俊云 唐利洲[1] 刘潮[1] WANG Haibo;GUO Junyun;TANG Lizhou;LIU Chao(Qujing Normal University,Center for Yunnan Plateau Biological Resources Protection and Utilization,Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution forAnimals and Plants on Yungui Plateau,Qujing 655011,China;College of Biological Resource and FoodEngineering,Qujing Normal University,Qujing 655011,China)

机构地区：[1]曲靖师范学院云南高原生物资源保护与利用研究中心云南省高校云贵高原动植物遗传多样性及生态适应性进化重点实验室,云南曲靖655011 [2]曲靖师范学院生物资源与食品工程学院,云南曲靖655011

出　　处：《华北农学报》2019年第2期44-58,共15页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31460179;31460561)

摘　　要：为明确小桐子bZIP转录因子家族的结构特征以及其在植物响应生物与非生物胁迫中的作用,利用生物信息学方法,从全基因组水平鉴定小桐子bZIP转录因子家族,并对其基因结构、进化关系、染色体定位、共线性关系及组织与低温表达特性进行分析。结果表明,共鉴定到51个小桐子bZIP基因,系统进化树分析将其分为10个亚族(A-I及S)。基因定位显示,51个小桐子bZIP基因不均匀地分布于11条染色体,其中2号与4号染色体鉴定到基因的串联复制。基因结构分析表明,小桐子bZIP基因包含1～13个外显子,其中,S亚族基因包含1～2个外显子,而G亚族基因包含12～13个外显子。bZIP转录因子主要定位在细胞核,氨基酸数目为113～768个,等电点4.70～10.30。启动子顺式作用元件鉴定发现3～27个响应激素如赤霉素、脱落酸、乙烯及生长素与非生物胁迫如低温、高温及创伤等调控元件。转录组表达分析显示,小桐子bZIP基因具有器官表达特异性,26个bZIP基因在叶片、根及种子中均有表达,其他基因仅在特定器官中表达。同时,鉴定到14个小桐子bZIP基因在低温条件下上调表达。在叶片中,qRT-PCR试验显示JcbZIP3与JcbZIP14表达量在低温处理24 h时上调达到显著水平,与小桐子抗冷性的形成及低温信号转导过程直接相关。研究结果为小桐子bZIP基因的克隆与调控机制研究奠定了基础。The purpose of the study was to further understand the structure characteristics of Basic leucine zipper (bZIPs) and its function in response to biotic and abiotic stress.Based on the Jatropha curcas genome and bioinformatics methods,bZIP family genes from J.curcas were identified,and then the gene structure,phylogentic relationship,chromosomal location,synteny relationship,as well as organ and chilling hardening expression,were systematically analyzed.The results showed that a total of 51 bZIP genes were systematically identified from J.curcas genome and classified into 10 subfamilies (A-I and S) according to phylogentic relationship.Chromosome mapping analysis indicated that J.curcas bZIP genes were distributed with different densities on 11 chromosomes,and tandem duplications were found on chromosome No.2 and 4,which was the main power for the expansion of J.curcas bZIP gene family.The results of gene structure analysis revealed that most of the J.curcas bZIP gene contained 1-13 exons,and the member of subfamilies of S and G owned 1-2 and 12-13 exons,respectively.Subcellular localization showed that the predicted bZIP proteins mainly located in nucleus with amino acid number ranged from 113 to 768 aa and pI value distributed from 4.70 to 10.30.3-27 hormonal response elements for gibberellin,abscisic acid,ethylene,auxin and abiotic stress response elements like low temperature,heat and wound were identified in the promoter of J.curcas bZIP genes.Transcriptome expression analysis showed that J.curcas bZIP genes exhibited different tissue-specific expression patterns,and 26 J.curcas bZIP genes were found to be expressed in the detected organs of leaves,roots,and seeds,while the others were expressed in the specific organ tissues.Moreover,the expression profiling of 14 J.curcas bZIP genes were up-regulated under chilling hardening.In leaves,JcbZIP3 and JcbZIP14 reached to the highest expression levels after 24 h chilling hardening based on the qRT-PCR results,which were associated with the events of chilling resis

关 键 词：小桐子 转录因子 BZIP 基因家族 表达分析 

分 类 号：Q78[生物学—分子生物学] S794.03[农业科学—林木遗传育种]