地方特色金针菜品种大乌嘴提纯复壮技术研究  

Study on the purification and rejuvenation technology in daylily Da Wu Zui with local characteristics

作　　者：周玲玲[1] 张黎杰[1] 田福发[1] 姜若勇[1] ZHOU Lingling;ZHANG Lijie;TIAN Fufa;JIANG Ruoyong(Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China)

机构地区：[1]江苏省农业科学院宿迁农业科学研究所,江苏宿迁223800

出　　处：《北方农业学报》2019年第2期111-114,共4页Journal of Northern Agriculture

基　　金：江苏省农业科技自主创新基金项目[CX(16)1022];宿迁市2018年农业科技自主创新项目(SQCX2018-04)

摘　　要：以大乌嘴金针菜的幼嫩茎尖作为外植体进行离体培养,成功建立大乌嘴组培快繁体系。结果表明:不同激素种类及质量浓度对大乌嘴初代培养、继代培养和生根培养影响差异显著。诱导愈伤组织的最适激素浓度为6-BA 3 mg/L和NAA 0.4 mg/L;诱导不定芽的最适激素浓度为6-BA 3 mg/L和NAA 0.2 mg/L;诱导生根的最适激素浓度为NAA0.2 mg/L或IBA0.2 mg/L。该研究为金针菜品种大乌嘴大规模生产提供科学依据。The shoot tip of daylily Da Wu Zui was used to establish techniques and a system for its tissue culture.Different hormone types and concentrations were examined in the study and it was found that these had significant effects on primary culture,starting culture,subculture and rooting culture of Da Wu Zui.The most suitable hormone concentration for callus Induction was MS supplemented with 3 mg/L 6-BA and 0.4 mg/L NAA.The most suitable hormone concentration for induction of adventitious buds was MS supplemented with 3 mg/L 6-BA and 0.2 mg/L NAA.The most suitable hormone concentration for root formation was 1/2MS with 0.2 mg/L NAA or 0.2 mg/L IBA.This study provides a scientific basis for the large-scale production of daylily Da Wu Zui.

关 键 词：金针菜 组织培养 培养基 

分 类 号：S682.19[农业科学—观赏园艺]