天台县汉坦病毒宿主动物调查及病毒分离结果  

The host investigation and virus isolation of hantavirus in Tiantai County

作　　者：姚苹苹[1] 徐芳[1] 朱函坪[1] 杨章女[1] 孙一晟[1] 卢杭景 陈晨 庞卫龙[2] 张云[3] 项海青 YAO Ping-ping;XU Fang;ZHU Han-Ping;YANG Zhang-nyu;SUN Yi-sheng;LU Hang-jing;CHEN Chen;PANG Wei-long;ZHANG Yun;XIANG Hai-qing(Key Lab. of Vaccine,Prevention and Control of Infectious Disease of Zhejiang Province,Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China)

机构地区：[1]浙江省疾病预防控制中心,浙江省传染病疫苗与预防控制研究重点实验室,浙江杭州310051 [2]天台县疾病预防控制中心 [3]南京军区军事医学研究所 [4]杭州市卫生事业发展中心

出　　处：《预防医学》2019年第5期433-436,440,共5页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：国家自然科学基金(81473036;81171609)

摘　　要：目的了解2011—2018年浙江省天台县汉坦病毒(HV)宿主动物的种群分布、带毒情况和病毒型别,为防制肾综合征出血热(HFRS)提供依据。方法在天台县HFRS监测点采用夹夜法捕获鼠形动物,对鼠种和鼠龄进行鉴定,分析当地鼠种构成和鼠密度。取鼠肺和鼠血,采用免疫荧光法检测HV抗原和抗体,HV抗原阳性鼠肺用HV S片段特异性引物进行RT-PCR核酸检测,并进行分离病毒和鉴定型别。结果 2011—2018年天台县平均鼠密度为4.44%,野外鼠密度为4.94%,居民区鼠密度为2.23%。捕获鼠形动物10种,野鼠优势鼠种为黑线姬鼠,家鼠优势鼠种为褐家鼠。鼠肺HV抗原阳性67份,阳性率为4.13%,其中1份来自褐家鼠,其他66份均来自黑线姬鼠;鼠血抗体阳性79份,阳性率为4.86%,均来自黑线姬鼠。67份HV抗原阳性鼠肺标本经RT-PCR扩增后,阳性34份,阳性率为50.75%。分离到22株HV,其中汉滩型(HTN) 21株,汉城型(SEO) 1株,均分离自黑线姬鼠。结论天台县存在以黑线姬鼠为主要传染源的HFRS疫源地,主要流行型别为HTN型,同时首次在天台县发现黑线姬鼠携带SEO型病毒。Objective To learn the population,virus status and viral types of hantavirus(HV)hosts in Tiantai County of Zhejiang Province from 2011 to 2018,and to provide evidence for hemorrhagic fever with renal syndrome(HFRS)control.Methods Rodents in Tiantai County were captured by night trapping method.After the species and age of rodents were identified,the composition of rodent species,dominant species and density of rodents were analyzed.The lungs and blood of rodents were sampled to detect the antigen and antibody of HV by immunofluorescence method.The HV antigen-positive lung samples were detected by RT-PCR with specific primers of HV S fragment,then HV was isolated and identified by inoculating Vero-E6 cells.Results The average rodent density in Tiantai County from 2011 to 2018 was 4.44%.The rodent density in the field and residential areas were 4.94% and 2.23%,respectively.Ten species of rodents were identified,with Apodemus agrarius dominant in the field and Rattus norvegicus in the residential areas.Sixty-seven lung samples were HV antigen positive(4.13%),one from Rattus norvegicus and the other sixty-six from Apodemus agrarius. Seventy-nine blood samples were HV antibody positive(4.86%),all from Apodemus agrarius.Thirty-four HV antigen-positive lung samples were positive(50.75%)after RT-PCR amplification.Twenty-two strains of virus were isolated and all of them were from Apodemus agrarius,including twenty-one strains of Hantaan type(HTN)and one strain of Seoul type(SEO).Conclusion In Tiantai County,Apodemus agrarius is the main source of HFRS infection;the main epidemic type of HV is HTN and SEO is first found in Apodemus agrarius.

关 键 词：汉坦病毒 肾综合征出血热 汉城型 鼠密度 

分 类 号：R512.8[医药卫生—内科学]