DNA条形码技术鉴定浙江省常见鼠形动物研究  

Application of DNA barcoding to identification of rodents in Zhejiang Province

作　　者：侯娟[1] 李天奇 丁春喜[2] 刘钦梅 张蓉[1] 黄健[2] 王金娜[1] 吴瑜燕[1] 龚震宇[1] HOU Juan*;LI Tian-qi;DING Chun-xi;LIU Qin-mei;ZHANG Rong;HUANG Jian;WANG Jin-na;WU Yu-yan;GONG Zhen-yu(Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China)

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051 [2]嘉善县疾病预防控制中心

出　　处：《预防医学》2019年第5期437-440,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：浙江省预防医学会课题(2017YF13);国家科技重大专项课题(2017ZX10303404)

摘　　要：目的利用DNA条形码技术鉴定浙江省常见鼠形动物。方法在浙江省嘉善县、龙游县、云和县和宁海县捕获野外鼠形动物,取鼠耳标本提取DNA,采用通用引物PCR扩增线粒体细胞色素C氧化酶亚基I (COI)基因测序。将测序获得序列与Gen Bank中的序列进行Blast序列同源性比对,采用邻接法构建分子进化树鉴定鼠形动物,并与传统形态学方法鉴定结果进行比较。结果共扩增出22份COI标本,其中18份标本构建的进化树与传统形态学鉴定结果一致;3份标本的鉴定结果存在差异,臭鼩鼱(Suncus murinus)重新鉴定为大麝鼩(Crocidura lasiura),幼年黄毛鼠(Rattus losea)、幼年黄胸鼠(Rattus tanezumi)重新鉴定为幼年褐家鼠(Rattus rattus);1份形态学鉴定为幼年东方田鼠(Microtus fortis)的标本(编号:NH-1)在Gen Bank中同源性较低,有待进一步鉴定。结论 DNA条形码技术能有效更正传统形态学鉴定可能造成的错误,两种方法联合可提高鼠型动物鉴定的准确性。Objective To apply DNA barcoding to identifying the rodents in Zhejiang Province.Methods Rodents were captured from Jiashan,Longyou,Yunhe and Ninghai counties in Zhejiang Province.The DNA was extracted from ears of rodent samples,and was amplified and sequenced with mitochondrial cytochrome C oxidase subunit I(COI)genes.The obtained sequences were compared with the related sequences in GenBank,and neighbour-joining evolutionary tree was constructed.Then the results by DNA barcoding and by morphological identification were compared.Results A total of 22 COI gene samples were amplified.The evolutionary tree constructed by 18 samples was consistent with the morphological identification results and 4 samples were different:Suncus murinus should be Crocidura lasiura,infant rats of Rattus losea and Rattus tanezumi was re-identified as Rattus rattus,infant rats of Microtus fortis(sample number:NH-1)needs further identification.Conclusion DNA barcoding can effectively correct the errors of morphological identification,thus combining the two methods could improve the accuracy of rodent identification.

关 键 词：鼠形动物 DNA条形码 线粒体细胞色素C氧化酶亚基I基因 分类鉴定 

分 类 号：R332[医药卫生—人体生理学]