响应面法优化巴戟天多糖提取工艺  

Optimization of extraction of poiysaccharide from Morinda officinalis by response surface methodology

作　　者：蔡月琴[1,2] 刘艺敏 凡莉莉 杜溶讫 荣俊冬 郑郁善[1] CAI Yueqin;LIU Yimin;FAN Lili;DU Rongqi;RONG Jundong;ZHENG Yushan(College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;College of Biological Sciences and Technology, Minnan Normal University, Zhangzhou, Fujian 363000, China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]闽南师范大学生物科学与技术学院,福建漳州363000

出　　处：《森林与环境学报》2019年第3期303-309,共7页Journal of Forest and Environment

基　　金：国家科技支撑计划项目(2009BAI73B01);福建省教育厅项目(JAT170359);漳州市现代种业发展项目"巴戟天种质资源保护与利用"

摘　　要：为确定巴戟天多糖热水浸提的最佳工艺,以多糖提取率为考察指标,选取液料比、浸提时间、浸提温度3个因素进行单因素试验和基于Box-Behnken设计的响应面试验,利用Design-Expert软件对多糖提取率的二次多项式回归模型进行分析,结果表明:浸提时间对巴戟天多糖提取率影响最为显著,巴戟天多糖提取的最佳工艺为液料比为25∶1 m L·g^(-1),浸提温度为81℃,浸提时间为3.1 h;该条件下多糖提取率为11.282 4%,与模型预测值接近,相对误差仅为0.38%,说明采用响应面法优化巴戟天多糖提取工艺合理可行。To optimize the hot water extraction process of polysaccharides from Morinda officinalis by response surface method, single factor experiment was investigated firstly and then based on the single factor experiment, a three factors and three levels Box-Behnken experiment was designed, in which extraction temperature and time, liquid-material ratio were chosen as independent variable, and Design-Expert software was applied to analyse the quadratic polynomial model of polysaccharide yield. The results showed extraction time had the most significant effect on polysaccharide yield and the optimum conditions of hot water extraction were determined as follows : liquid-solid ratio of 25∶1 mL · g ^-1 , extraction temperature of 81 ℃, extraction time of 3.1 h. In this condition, the polysaccharide yield was up to 11.282 4% which was close to the predicted value, and the relative error among them was 0.38%, suggesting the applicability of response surface methodology to optimize the polysaccharide extraction process from Morinda officinalis .

关 键 词：巴戟天 多糖 提取工艺 响应面分析法 

分 类 号：S567.19[农业科学—中草药栽培]