青光安有效组分对兔青光眼术后滤过道瘢痕组织中胶原纤维、成纤维细胞特异性蛋白-1、结缔组织生长因子及转化生长因子-β1表达的影响  

Effects of Effective Components of Qingguang'an on Collagen Fibers, Fibroblast-specific Protein-1, Connective Tissue Growth Factor and Transforming Growth Factor-β1 in Scarring of Filtering Passage After Glaucoma Surgery of Rabbits

作　　者：蒋鹏飞[1,2] 黄学思 彭俊[1,3] 李苑碧[1,2] 喻娟 彭清华 JIANG Pengfei;HUANG Xuesi;PENG Jun;LI Yuanbi;YU Juan;PENG Qinghua(Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Provincial Key Laboratory of Otorhinolaryngology and Laryngeal Diseases,Changsha 410208,China;The First Affiliated Hospital of Hunan College of Chinese Medicine,Zhuzhou 412000,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,湖南长沙410208 [3]湖南中医药高等专科学校附属第一医院,湖南株洲412000

出　　处：《中国中医药信息杂志》2019年第5期59-63,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81273807;81603665);教育部博士学科点基金(20124323110006);湖南省自然科学基金重点项目(11JJ2050;2017JJ3238);中国博士后科学基金(2017M612565);湖南省研究生创新基金重点资助项目(CX2013A013);国家中医药管理局中医眼科学重点学科建设项目(ZK1801YK015);湖南省高层次卫生人才"225"工程培养项目(2013年);湖南中医药大学中医学国内一流建设学科(2018-2020年)

摘　　要：目的观察青光安4种有效组分对兔青光眼术后滤过道瘢痕组织中胶原纤维、成纤维细胞特异性蛋白-1(FSP-1)、结缔组织生长因子(CTGF)、转化生长因子-β1(TGF-β1)表达的影响,对比青光安4种有效组分及青光安混悬液抗滤过道瘢痕化的效果。方法采用青光眼滤过手术制作动物模型。实验兔按体质量随机分为空白组(A组)、模型组(手术对照组,B组)、丝裂霉素C组(C组)、有效组分1组(D组)、有效组分2组(E组)、有效组分3组(F组)、有效组分4组(G组)和青光安混悬液组(H组)。各给药组给予相应药物。观察各组兔滤过道瘢痕组织中胶原纤维、FSP-1、CTGF、TGF-β1水平。结果术后C、E、H组兔眼压回升缓慢,眼压值较术前和A组差异均有统计学意义(P<0.05);胶原纤维面积比值除B、D、G组外,其他各组间比较差异均有统计学意义(P<0.05);FSP-1表达除B、G组外,其他各组间比较差异均有统计学意义(P<0.05);CTGF表达除B、D、G组外,其他各组间比较差异均有统计学意义(P<0.05);TGF-β1表达除B、G组外,其他各组间比较差异均有统计学意义(P<0.05)。结论青光安有效组分2、丝裂霉素C及青光安混悬液通过抑制胶原纤维、FSP-1、CTGF、TGF-β1的表达,降低肌成纤维细胞和成纤维细胞的增殖,从而减少滤过道瘢痕组织的增生,有明显抗青光眼术后滤过道瘢痕化的作用,青光安有效组分2和青光安混悬液抑制滤过道瘢痕化效果基本相当。Objective To observe the effects of effective components of Qingguang'an on collagen fibers,fibroblast-specific protein-1(FSP-1),connective tissue growth factor(CTGF)and transforming growth factor-β1(TGF-β1)in rabbits after glaucoma surgery;To compare the four effective components of Qingguang'an and the effects of Qingguang'an suspension on scarring of filtration passage.Methods Glaucoma filtering surgery was used to make animal models.The rabbits were randomly divided into blank group(group A),model group(surgical control group,group B)and mitomycin C group(group C),effective component 1 group(group D),effective component 2 group(group E),effective component 3 group(group F),effective component 4 group(group G)and Qingguang'an suspension group(group H)according to body weight.Each administration group was given relevant medicine.The levels of collagen fibers,FSP-1,CTGF and TGF-β1 in the scar tissue of rabbit filtration passage in each group were observed.Results After surgery,the intraocular pressures of group C,E and H were slow,with statistical significance compared with preoperation and group A,(P<0.05).The area ratio of collagen fibers was statistically significant except for the comparison among group B,D and G(P<0.05).The expression of FSP-1 was statistically significant among the other groups except for the comparison between group B and group G(P<0.05).The expression of CTGF was statistically significant among the other groups except for the comparison among group B,D and G(P<0.05).The expression of TGF-β1 was statistically significant among the other groups except for the comparison between group B and G group(P<0.05).Conclusion Effective component 2 in Qingguang'an,mitomycin C and Qingguang'an suspension can reduce the proliferation of myofibroblasts and fibroblasts by inhibiting the expressions of collagen fibers,FSP-1,CTGF and TGF-β1,and show obvious anti-glaucoma scarring effect after filtration.The efficacy of effective component 2 in Qingguang'an and Qingguang'an suspension for inhibiting th

关 键 词：青光安有效组分 青光眼 滤过道瘢痕化 胶原纤维 成纤维细胞特异性蛋白-1 结缔组织生长因子 转化生长因子-β1 兔 

分 类 号：R285.5[医药卫生—中药学]