藿香正气制剂中和厚朴酚及总多糖快速测定分析  

Rapid Determination and Analysis of Honokiol and Total Polysaccharide in Huoxiang Zhengqi Preparations

作　　者：朱俊平[1,2,3] 梁慧慧 文雯 毛妍 范诗琪 刘文龙[1,2,3] 贺福元[1,2,3] 张喜利 ZHU Junping;LIANG Huihui;WEN Wen;MAO Yan;FAN Shiqi;LIU Wenlong;HE Fuyuan;ZHANG Xili(Pharmacy College,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine,Changsha 410208,China;Supramolecular Mechanism and Mathematic-Physics Characterization for Chinese Materia Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]中药成药性与制剂制备湖南省重点实验室,湖南长沙410208 [3]湖南中医药大学中医药超分子机理与数理特征化实验室,湖南长沙410208

出　　处：《中国中医药信息杂志》2019年第5期78-82,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81270055;81874344);国家中药标准化项目(ZYBZH-Y-HUN-24);湖南省自然科学基金(2019JJ40220);中国博士后基金(2018M640755);湖南省中医药科研计划项目(201811);湖南中医药大学"一方研究生科研创新项目"(YF201705)

摘　　要：目的建立HPLC双波长指纹图谱快速测定藿香正气制剂中和厚朴酚含量的方法,建立糖量计法快速测定其总多糖含量的方法,并分析其差异性。方法 HPLC采用XB-C18(HS)色谱柱(4.6 mm×250 mm,5μm),乙腈和0.1%磷酸水溶液等度洗脱,流速1.0 mL/min,进样量20μL,检测波长294、270 nm,柱温30℃;糖量计法以50%乙醇为阴性对照;聚类分析重复性样品,对比不同指标间的聚类结果。结果 HPLC测得和厚朴酚在1.40～0.14 mg/mL范围内与峰面积线性关系良好,线性方程为A=12182633C-217418,r^2=0.9995;藿香正气水中和厚朴酚浓度为(0.4008±0.0050)mg/mL,藿香正气滴丸中其浓度为(0.4879±0.0089)mg/mL。总多糖在0.91～0.29 g/mL范围内与其百分值线性关系良好,线性方程为Y=51.949X+0.868,r^2=0.9999;藿香正气水中总多糖浓度为(0.31±0.003)g/mL,藿香正气滴丸中其浓度为(1.12±0.005)g/mL。聚类分析筛选出和厚朴酚峰面积和总多糖百分值作为表现藿香正气水和藿香正气滴丸间差异的指标。结论本研究建立的HPLC及糖量计法能有效用于藿香正气制剂多类指标的质量控制及不同剂型间的差异考察;聚类分析筛选出的峰面积和总多糖百分值作为评价指标具有代表性和准确性。Objective To establish a method for rapid determination of honokiol in Huoxiang Zhengqi Preparations by HPLC dual-wavelength fingerprint,and to establish a sugar meter for rapid determination of total polysaccharide in Huoxiang Zhengqi Preparations,and to analyze its differences.Methods HPLC was performed on an XB-C18(HS)column(4.6 mm×250 mm,5μm),with acetonitrile and 0.1%phosphoric acid aqueous solution for isocratic elution.The flow rate was 1.0 mL/min;the injection volume was 20μL;the detection wavelength was 294 and 270 nm;the column temperature was 30℃.Sugar meter method was performed with 50%ethanol as negative control.Cluster analysis was conducted to analyze repeated samples and comparison of clustering results among different indexes.Results The linear relationship between the concentration of honokiol and the peak area in the range of 1.40-0.14 mg/mL was good by HPLC.The linear equation was A=12 182 633C-217 418,r^2=0.999 5.The concentration of honokiol in Huoxiang Zhengqi Liquid was(0.400 8±0.005 0)mg/mL;the concentration of honokiol in Huoxiang Zhengqi Dropping Pills was(0.487 9±0.008 9)mg/mL.The linear relationship between the concentration of total polysaccharide and its percentage was good in the range of 0.91–0.29 g/mL;the linear equation was Y=51.949X+0.868,r^2=0.999 9.The concentration of total polysaccharide in Huoxiang Zhengqi Liquid was(0.31±0.003)g/mL;the total polysaccharide in Huoxiang Zhengqi Dropping Pills was(1.12±0.005)g/mL.The area of honokiol peak and the percentage of total polysaccharide as indicators of Huoxiang Zhengqi Liquid and Huoxiang Zhengqi Dropping Pills showed significant differences between different formulations.Conclusion Established HPLC and sugar meter method can be effectively used for the quality control and investigation of differences between different dosage forms of Huoxiang Zhengqi Preparations;the selected peak area and total polysaccharide percentage as the evaluation index are representative and accurate.

关 键 词：藿香正气水 藿香正气滴丸 高效液相色谱法 和厚朴酚 总多糖 快速测定 聚类分析 

分 类 号：R284.1[医药卫生—中药学]