羟丙基化碱木质素的制备及其对纤维素酶水解的影响  

Preparation of Hydroxypropylated Alkali Lignin and Its Effect on Hydrolysis Efficiency of Cellulase

作　　者：赵存异 应文俊 史正军[1,2] 杨海艳 郑志锋 杨静 ZHAO Cunyi;YING Wenjun;SHI Zhengjun;YANG Haiyan;ZHENG Zhifeng;YANG Jing(Key Laboratory for Forest Resources Conservation and Utilisation in the Southwest Mountains of China,Ministry of Education,Southwest Forestry University,Kunming 650224,China;College of Chemical Engineering,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南山地森林资源保育与利用教育部重点实验室西南林业大学,云南昆明650224 [2]西南林业大学化学工程学院,云南昆明650224

出　　处：《林产化学与工业》2019年第2期109-114,共6页Chemistry and Industry of Forest Products

基　　金：云南省农业基础研究联合专项(2017FG001(-025));国家自然科学基金资助项目(31760194)

摘　　要：以碱木质素(AL)为原料制备羟丙基化碱木质素(HL),研究HL对纤维素酶的非生产性吸附性能的影响机制,并进一步探讨其对纤维素的酶水解得率的影响。Zeta电位滴定、X射线光电子能谱以及疏水性的测试结果表明:AL经过羟丙基化改性后表面特性发生改变,表面负电荷增加(Zeta电位由+35.0 mV降至-44.8 mV);表面元素分布及化学键组成发生了较大的变化,C—O和■键强度增加,疏水性减弱(疏水度由106.60 L/g减小为4.30 L/g),使得木质纤维素底物对纤维素酶的非生产性吸附减弱,进而显著提高纤维素酶水解效率。以10 U/g纤维素酶水解0.4 g/L微晶纤维素72 h,添加4 g/L的HL时游离酶蛋白质量分数为11.65%,相比4 g/L的AL提高152%;添加4 g/L的HL时酶水解得率为54.38%,相比4 g/L的AL提高32.09%。Alkali lignin (AL) was modified by hydroxypropylationto obtain hydroxypropylated lignin (HL), and its effects on enzymatic hydrolysis and non-productive enzyme adsorption were further investigated. The results of Zeta potentials, X-ray photoelectron spectroscopy and hydrophobicity tests indicated that the surface charaoteristics of AL changed after hydroxypropylation. The surface negative charge increased and the surface elements distribution and chemical bonds composition changed greatly. The proportion of C-O and C-C ═O bond increased, as well as the hydrophobicity decreased, resulting in the reducing of non-productive adsorption of cellulase onto HL, which significantly increased the enzymatic hydrolysis efficiency. Compared with these of Avicel with AL, the free enzyme protein of Avicel with 4 g/L HL increased by 152 % and the 72 h hydrolysis yield of glucose increased by 32.09%.

关 键 词：碱木质素 羟丙基化改性 表面特性 纤维素酶水解 

分 类 号：TQ35[化学工程]