安神方颗粒对CUMS大鼠递质代谢与炎症反应的影响  

Effect of Anshenfang granules on neurotransmitter metabolism and inflammatory response in rats with chronic unpredictable mild stress

作　　者：聂玲辉 黄杰聪 谢炜[3] 伍志勇[3] NIE Linghui;HUANG Jiecong;XIE Wei;WU Zhiyong(Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute, Guangzhou, 510317, China;Guangzhou Conghua District Hospital of Traditional Chinese Medicine, Guangzhou, 510900, China;School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China)

机构地区：[1]广东省第二人民医院传统医学与运动伤害康复研究所,广东广州510317 [2]广州市从化区中医院,广东广州510990 [3]南方医科大学中医药学院,广东广州510515

出　　处：《暨南大学学报（自然科学与医学版）》2019年第2期135-142,共8页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学基金项目(2017A030310289);广东省中医药科研项目(20182090)

摘　　要：目的:探讨安神方颗粒对慢性应激抑郁模型大鼠行为学以及递质代谢和炎症反应的影响.方法:30只Wistar大鼠随机分为空白组、模型组、安神方颗粒组,每组10只;除空白组外,其余各组大鼠进行2周的慢性温和不可预知应激(CUMS)刺激;在后续3周进行CUMS 1 h前,安神方颗粒组每天给予安神方颗粒质量分数40 g/kg灌胃,空白组和模型组给予等体积生理盐水灌胃.观察各组大鼠治疗前后的体质量变化;运用强迫游泳(FST)和糖水偏好实验(SPT)评估大鼠抑郁行为的变化;通过ELISA法检测大鼠海马组织中炎症因子白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的含量,并通过Western blotting和免疫荧光检测大鼠海马BDNF和GABA_ARα2表达情况.结果:与空白组比较,模型组大鼠体质量增加缓慢(P <0. 01),FST累积不动时间明显延长(P <0. 01),糖水偏好值减少(P <0. 01),海马BDNF和GABA_ARα2表达水平下降(P <0. 01);与模型组比较,安神方颗粒组大鼠体质量增长较快(P <0. 01),FST累积不动时间明显缩短(P <0. 01),糖水偏好值升高(P <0. 01),海马组织中炎症因子IL-1β和TNF-α的含量降低,BDNF和GABA_ARα2表达水平上升(P <0. 01).结论:安神方颗粒可能参与干预炎症因子IL-1β和TNF-α上调海马组织BDNF和GABA_ARα2的表达,从而发挥抗抑郁的作用.Objective: To explore the effect of Anshenfang granules( ASF) on behavior,neurotransmitter metabolism and inflammatory response in chronic stress depression model rats.Methods: Thirty Wistar rats were randomly divided into control group,model group and ASF group with10 rats in each group. Except control group,the rats of the other two group were stimulated by chronic unpredictable mild stress( CUMS) for 2 weeks. In the following 3 weeks,ASF group was intragastrically given 40 g/kg ASF at 1 h before CUMS,while control and model group were given equal volume of normal saline. The changes of body weight before and after treatment were observed. The depressive behavior of rats was assessed by forced swimming test( FST) and sugar-water preference test( SPT).The level of inflammatory factors IL-1β and TNF-α_in hippocampus of rats was detected by ELISA,and the level of BDNF and GABA_ARα-2 in hippocampus were detected by Western blotting and immunofluorescence method. Results: Compared with the control group,the weight gain of rats in the model group was slow( P < 0. 01),the accumulative immobility time of FST was significantly prolonged( P < 0. 01),the preference value of sugar and water was decreased( P < 0. 01),and the expression level of BDNF and GABA_ARα-2 in hippocampus was decreased( P < 0. 01). Compared with the model group,the weight gain of rats in ASF group was faster( P < 0. 01),the accumulative immobility time of FST was significantly shortened( P < 0. 01),the preference value of sugar and water was increased( P < 0. 01),the content of inflammatory factors IL-1β and TNF-α_in hippocampus was decreased,and the expression level of BDNF and GABA_ARα-2 was increased( P < 0. 01). Conclusion: ASF may be involved in the intervention of inflammatory factors IL-1β and TNF-α_to up-regulate the expression of BDNF and GABA_ARα-2 in hippocampus,thus playing an anti-depression role.

关 键 词：抑郁 安神方颗粒 慢性温和不可预知刺激 递质代谢 炎症反应 

分 类 号：R277.7[医药卫生—中医学]