载黑磷量子点脂质体用于宫颈癌光热治疗的体外研究  

In vitro study of black phosphorus quantum dot-loaded liposomes for photothermal therapy of cervical cancer

作　　者：陈秀丽 周韵 梁欣 梅林[1,2] 武小金 CHEN Xiu-li;ZHOU Yun;LIANG Xin;MEI Lin;WU Xiao-jin(School of Life Science, Tsinghua University, Beijing 100084, China;School of Pharmaceutics Science(Shenzhen), Sun Yat-sen University, Guangzhou 510275, China)

机构地区：[1]清华大学生命科学学院,北京100084 [2]中山大学药学院(深圳),广东广州510275

出　　处：《药学学报》2019年第4期729-736,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81771966);广东杰出青年科学基金资助项目(2014A030306036)

摘　　要：本研究制备载黑磷量子点(BPQDs)脂质体(liposome-BPQDs),探究其理化性质及用于宫颈癌光热治疗的效果。应用超声法制备BPQDs,薄膜分散法制备liposome-BPQDs,并对其形貌、粒径、电位和拉曼光谱等进行表征。采用CCK-8法检测该纳米粒对人宫颈癌细胞(HeLa)的毒性。使用激光共聚焦显微镜(CLSM)和荧光倒置显微镜分别观察HeLa细胞摄取和细胞凋亡情况。结果表明,扫描电镜下, liposome-BPQDs呈椭球状或球状;透射电镜观察显示liposome-BPQDs粒径约90～110 nm。粒径及电位测量结果表明liposome-BPQDs粒径为(104.2±0.35) nm, zeta电位(-11.3±3.01) mV。脂质体包封率为(84.40±2.13)%。在室外通风、温度范围25℃～34℃和相对湿度80%～82%自然条件下, liposome-BPQDs光热效应良好,降解较BPQDs缓慢。Liposome-BPQDs可被HeLa细胞所摄取;近红外激光照射后,载BPQDs量达20μg·mL^(-1)时, HeLa细胞死亡率大幅度上升。本研究表明, liposomeBPQDs稳定性较高,且具有良好的光热效应,有望应用于宫颈癌光热治疗。In this study, black phosphorus quantum dots (BPQDs)-loaded liposomes (liposome-BPQDs) were prepared to explore physicochemical properties and photothermal effects on cervical cancer cells. BPQDs were fabricated by ultrasonic method. Liposome-BPQDs were prepared by thin film dispersion. Surface morphology, particle size, zeta potential and Raman spectra of liposome-BPQDs were characterized. The cytotoxicity of the liposome-BPQDs against human cervical cancer cells (HeLa) was examined by CCK-8 assay. Confocal laser scanning microscope (CLSM) and fluorescence microscopy were used to observe the uptake and apoptosis of HeLa cells. The results indicated that liposome-BPQDs were ellipsoidal or spherical under scanning electron microscope, TEM observation showed liposome-BPQDs were about 90-110 nm in diameter. The particle size measurements showed liposome-BPQDs were (104.2±0.35) nm in diameter, and zeta potential were examined to be (-11.3±3.01) mV. The encapsulation efficiency was (84.40±2.13)%.Under natural conditions with outdoor ventilation, temperature range of 25℃-34℃ and relative humidity of 80%-82%, the photothermal effects of liposome-BPQDs was better and the degradation denaturation of liposome-BPQDs were slower than those of BPQDs. The results also reflected that liposome-BPQDs could be uptaken by HeLa cells easily. After near-infrared laser irradiation, the mortality of HeLa cells rise significantly when the amount of BPQDs reach 20 μg·mL-1. In summary, liposome-BPQDs with high stability exhibited good photothermal effects, which can be expected to be applied to photothermal therapy of cervical carcinoma.

关 键 词：黑磷量子点 脂质体 理化性质 宫颈癌 光热治疗 

分 类 号：R943[医药卫生—药剂学]