我国不同流行区婴儿利什曼原虫前鞭毛体比较蛋白质组学分析  

Comparative proteomics analysis of Leishmania infantum strains isolated from two epidemiological regions of visceral leishmaniasis in China

作　　者：危芙蓉[1] 高春花[1] 汪俊云[1] 杨玥涛[1] 石锋[1] WEI Fu-Rong;GAO Chun-Hua;WANG Jun-Yun;YANG Yue-Tao;SHI Feng(National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention,Laboratory of Parasite and Vector Biology ,National Health Commission,National Center for International Research on Tropical Diseases, Ministry of Science and Technology,WHO Collaborating Centre for Tropical Diseases, Shanghai 200025, China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所国家卫生健康委员会病原与媒介生物学重点实验室科技部国家级热带病国际联合研究中心世界卫生组织热带病合作中心,上海200025

出　　处：《中国血吸虫病防治杂志》2018年第6期625-629,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81472923)

摘　　要：目的应用比较蛋白质组学方法分析两株来自我国内脏利什曼病不同流行区的婴儿利什曼原虫前鞭毛体蛋白表达差异,为筛选和鉴定我国不同流行区利什曼原虫致病差异相关分子奠定基础。方法分别培养两株分离于我国四川九寨沟县(SC6株)和新疆伽师县内脏利什曼病流行区(JIASHI-5株)的婴儿利什曼原虫前鞭毛体,经酶解后进行液相色谱串联质谱(Liquid chromatography-tandem mass spectrometry,LC-MS/MS)非标记(Label-free)定量分析,利用MaxQuant软件(版本号1.3.0.5)查库,进行非标定量(Label-free quantitation,LFQ)分析。结果成功鉴定蛋白4 274个,筛选出差异蛋白1 219个(差异倍数>2.0或<0.5,P<0.05),其中JIASHI-5株前鞭毛体特有蛋白550个,SC6株前鞭毛体特有蛋白174个。SC6株和JIASHI-5株间差异表达蛋白495个,其中SC6株上调蛋白(高表达)167个,JIASHI-5株上调蛋白328个。这些差异表达蛋白主要涉及能量代谢、应激反应、延长感染宿主细胞的寿命以及利什曼原虫存活与增殖。结论来自我国内脏利什曼病不同流行区的婴儿利什曼原虫前鞭毛体蛋白质表达存在差异。Objective To analyze the protein abundance differences between two Leishmania infantum strains isolated from different epidemiological types of visceral leishmaniasis in China by comparative proteomics method. Methods Tryptic digests of total proteins were analyzed by using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by label-free quantitative differential expression analysis. The MS data were analyzed with MaxQuant software (ver 1.3.0.5) against data base. Results This study resulted in the identification of 4 274 proteins across two strains (JIASHI-5 and SC6). Of these, 1219 differentially expressed proteins (ratio> 2.0 or<0.5 , P<0.05)were identified. Considering the proteins differentially or uniquely expressed in the strains, 550 proteins were only found in the JIASHI-5 strain, and 174 proteins were only found in the SC6 strain. Totally 495 differentially proteins were expressed in the two groups, among which 328 proteins were down-regulated and 167 proteins were up-regulated in SC6 strain. Some of the identified differentially expressed proteins were demonstrated and they involved in energy metabolism, stress response, prolonging the lifetime of the infected host cell and survival and proliferation in virulent strains. Conclusion This study reveals a group of differentially expressed proteins and the related biologic function that may lay the foundation for screening and identification of the key Leishmania molecules relative to pathogenicity.

关 键 词：利什曼原虫 内脏利什曼病 比较蛋白质组学 差异蛋白 非标记定量分析 

分 类 号：R382.22[医药卫生—医学寄生虫学]