RSM优化单宁酸络合镁的制备条件及抑菌性能研究  

Optimization of Preparation Conditions and Antibacterial Activities of Tannic Acid Complexing Magnesium by RSM

作　　者：解彩玲 史泽毅 卫钰茹 刘明 赵越 杨冬花[1] 靳利娥[1] XIE Cai-ling;SHI Ze-yi;WEI Yu-ru;LIU Ming;ZHAO Yue;YANG Dong-hua;JIN Li-e(College of Chemistry and Chemical Engineering,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]太原理工大学化学化工学院,山西太原030024

出　　处：《化学试剂》2019年第4期348-353,共6页Chemical Reagents

基　　金：国家自然科学基金项目(51174144)

摘　　要：探讨以单宁酸(TA)为原料与氯化镁在碱性的条件下进行络合反应制备单宁酸络合镁(T-Mg)的最佳合成条件,并研究了T-Mg的抑菌性能。在单因素实验的基础上,采用响应曲面法(RSM)对T-Mg制备条件进行优化,通过傅里叶红外光谱、紫外光谱、X-射线衍射表征其结构。响应面优化实验表明,物料比、反应温度、反应p H对T-Mg产率均有显著影响,优化得到的T-Mg最佳制备条件为:n(单宁酸)∶n(氯化镁)=1∶2. 70、反应温度为44℃、p H为8. 0,在该条件下T-Mg产率可达80. 95%。以大肠杆菌和金黄色葡萄球菌为供试菌,采用滤纸片法观察了T-Mg对供试菌的抑菌性能。结果表明,T-Mg对供试菌均有抑制作用,抑菌活性依次为金黄色葡萄球菌、大肠杆菌。T-Mg对供试菌的抑菌效果强于TA,并且随着T-Mg质量浓度的增加,抑菌效果增强。The aim was to optimize synthesis conditions and investigate antibacterial activities of tannic acid complexing magnesium.Tannic acid complexing magnesium was synthesized from tannic acid and magnesium chloride as raw materials under alkaline conditions.On the basis of single factor test,response surface methodology was applied to optimize the main synthesis conditions.The structure and properties were characterized by FT-IR,UV and XRD.The result showed that the optimum synthesis conditions of optimized tannic acid complexing magnesium were n(tannic acid):n(magnesium chlorice)=1:2.70,reaction temperature 44℃,and pH8.0.Under these optimized conditions,the yield of tannic acid complexing magnesium was 80.95%.The antibacterial properties of tannic acid complexing magnesium against Escherichia coll and Staphylococcus aureus were observed by the filter paper method.The results showed that tannic acid complexing magnesium had inhibitory effects on these tested strains,and the antimicrobial activities were Staphylococcus aureus>Escherichia coli.Tannic acid complexing magnesium had generally stronger antibacterial activities on these tested strains than that of tannic acid,and the antibacterial activities were enhanced with the increase of the mass concentration of tannic acid complexing magnesium.

关 键 词：单宁酸 单宁酸络合镁 响应曲面法 抑菌性能 

分 类 号：O629[理学—有机化学]