青蒿琥酯对糖尿病大鼠伤口皮肤TNF-α的影响  

Effects of artesunate on the expression of TNF-α in the diabetic skin wounds in rats

作　　者：冯洁 农晓琳[1,2,3,4] FENG Jie;NONG Xiao-lin(College of Stomatology,Guangxi Medical University,Guangxi Nanning 530021,China;Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction,Guangxi Nanning 530021,China;Guangxi Clinical Research Center for Craniofacial Deformity,Guangxi Nanning 530021,China;Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment,Guangxi Nanning 530021,China)

机构地区：[1]广西医科大学附属口腔医院,广西南宁530021 [2]广西口腔颌面修复与重建研究自治区级重点实验室,广西南宁530021 [3]广西颅颌面畸形临床医学研究中心,广西南宁530021 [4]颌面外科疾病诊治研究重点实验室(广西高校重点实验室),广西南宁530021

出　　处：《全科口腔医学电子杂志》2019年第10期9-10,共2页Electronic Journal of General Stomatology

基　　金：国家自然科学基金地区项目(81860726);国家自然科学基金项目(81060156);广西自然广西医疗卫生适宜技术开发与推广应用项目(S201688);广西科学研究与技术开发计划项目(桂科能14123006-2)

摘　　要：目的建立糖尿病大鼠模型后,背部皮肤制造伤口,局部涂抹0.5%青蒿琥酯(ART)干预,观察药效并免疫组化法检测伤口皮肤肿瘤坏死因子-α(TNF-α)的影响。方法选取SD大鼠20只,随机分为糖尿病盐水组、糖尿病ART组,适应性喂养后糖尿病组大鼠注射链脲佐菌素(STZ)诱导1型糖尿病,监测血糖,成功建模2周后,所有大鼠背部皮肤制造伤口,用药2周后处死,取背部皮肤行免疫组织化学法观察皮肤TNF-α变化。结果注射STZ后成功建立糖尿病大鼠模型,免疫组化提示糖尿病ART组伤口皮肤中TNF-α表达较糖尿病盐水组显著下降(P<0.05)。结论 ART能下降TNF-α的表达,通过抗炎作用,促进伤口愈合,为糖尿病皮肤伤口研究提供一种新的药物治疗方案。Objective To establish a diabetic rat model,the wounds were made on the back skin,and the 0.5% artesunate(ART) was applied.The effects of the drug and the tumor necrosis factor-α(TNF-α) expression in skin wounds were observed.Methods 20 SD rats were randomly divided into diabetic saline group and diabetic ART group.Diabetic rats were injected with streptozotocin (STZ) to induce type 1 diabetes,and blood glucose was monitored.After 2 weeks,wounds were made on the back skin of all rats.After 2 weeks of treatment,the rats were sacrificed.The effects of ART and the tumor necrosis factor-α(TNF-α) expression in skin wounds were observed. Results The diabetic rat model was successfully established.The expression of TNF-α in the diabetic ART group was significantly lower than that in the diabetic saline group (P<0.05).Conclusion ART cound exert antiinflammatory effect and be good for wound healing by decreasing the expression of TNF-α,and this provides a new drug treatment plan for diabetic skin wound research.

关 键 词：青蒿琥酯 1型糖尿病 皮肤伤口 TNF-Α 

分 类 号：R587.1[医药卫生—内分泌]