速度向量成像技术评价糖尿病大鼠腹主动脉弹性的研究  

The evaluation of elastic properties of abdominal aortic wall in rats with diabetes mellitus by velocity vector imaging

作　　者：曹丽[1] 郭丹丹 张凯[1] 李静波[3] 林静娜[3] Cao Li;Guo Dandan;Zhang Kai;Li Jingbo;Lin Jingna(Department of Geriatrics, Tianjin Medical University General Hospital, Tianjin 300052, China;Department of Medical Ultrasound, Tianjin 300052, China;Department of Endocrinology, Tianjin People's Hospital, Tianjin 300132, China)

机构地区：[1]天津医科大学总医院老年病科,300052 [2]天津医科大学总医院超声科,300052 [3]天津市人民医院内分泌科,300132

出　　处：《中华老年医学杂志》2019年第4期454-458,共5页Chinese Journal of Geriatrics

基　　金：天津市自然科学基金项目(12JCYBJC17700).

摘　　要：目的应用速度向量成像(VVI)技术评价2型糖尿病(DM)大鼠腹主动脉粥样硬化发生、发展中血管壁弹性功能的变化。 方法选择健康SD大鼠35只,高脂、高糖饲料喂养及注射链脲佐菌素建立DM大鼠模型,30只造模成功。分为0周(造模即刻)、4周、8周、12周和16周各6只。从0周开始,每隔4周对糖尿病大鼠进行常规超声观察腹主动脉的内中膜厚度(IMT)、收缩末期与舒张末期血管内径差值(ΔD)、阻力指数(RI)、搏动指数(PI)及血流收缩期峰值流速(Vs);应用VVI技术检测腹主动脉短轴的径向峰值速度(PRv),周向峰值应变(PCs),周向峰值应变率(PCsr)及径向峰值位移(PRd)。最后处死大鼠,采集腹主动脉标本,分别于光镜和电镜下观察腹主动脉不同时期的组织病理学改变及超微结构的变化。 结果造模16周与0周、4周比较,IMT增厚(0.30±0.03)mm比(0.25±0.03)mm、(0.26±0.02)mm,差异有统计学意义(F=3.718,P=0.017)。造模8周时PRv、PCs、PCsr、PRd低于0周大鼠,造模后16周时PRv、PCs、PCsr、PRd较造模后0周、4周及8周大鼠降低(F值分别为15.565,14.754,25.015,19.326,均P<0.01),12周和16周DM大鼠腹主动脉光镜下可见内膜基质层增厚,平滑肌细胞变形并向内膜迁移,泡沫细胞形成,电镜下可见内皮细胞有大量脂质附着,平滑肌细胞空泡变性。 结论VVI能够较二维超声更早的发现DM大鼠腹主动脉管壁弹性的变化,有利于分析血管壁的运动功能。Objective To evaluate the characteristics of arterial elasticity changes of abdominal aorta atherosclerosis in type 2 diabetic rats by using velocity vector imaging(VVI). Methods A rat model of diabetes mellitus(DM)were successfully established by a high-fat diet combined with the streptozotocin injection in thirty healthy male sprague-dawley rats.DM rats were randomly divided into five groups(n=6, each): 0-week group(rats before treatment), 4-week group(rats treated for 4 weeks), 8-week group(rats treated for 8 weeks), the 12-week group(rats treated for 12 weeks), the 16-week group(rats treated for 16 weeks). The intima-media thickness(IMT)of abdominal aorta, the difference of end-systolic and end-diastolic vessel diameter(ΔD), resistance index(RI), pulsatility index(PI)and peak systolic velocity(Vs)were detected by ultrasound every 4 weeks .Peak radial velocity(PRv), PCs rate(PCs), peak circumferential strain(PCsr) and peak radial displacement(PRd)of abdominal aorta in short axis view were determined by VVI technique.Rats were sacrificed in different stages.Then, the specimens of abdominal aorta were collected, and observed by light microscope and electron microscope for the histopathological and ultrastructural changes in the atherosclerosis, respectively. Results Compared with 0-week group(0.25±0.03)mm and 4-week group(0.26±0.02)mm, IMT was thickened in the 16 weeks group(0.30±0.03)mm,(F=3.718, P=0.017). PRv, PCs, PCsr and PRd were lower after 8 weeks of treatment than in 0-week group.PRv, PCs, PCsr and PRd were lower in 16-week group than 0-week, 4-week and 8-week groups(F=15.565, 14.754, 25.015 and 19.326, P<0.01). Under the light microscope in abdominal aorta of rats in 12-week group and 16-week group, the stromal layer was thickened, smooth muscle cells were deformed and migrated to the tunica intima, and foam cells were formed.And under electron microscope, endothelial cells had a large amount of lipid attachment, and smooth muscle cells had vacuolar degeneration. Conclusions As compared with the two

关 键 词：超声检查 主动脉 腹 动脉粥样硬化 

分 类 号：R587.2[医药卫生—内分泌] R445.1[医药卫生—内科学]