慢性低氧性肺动脉高压大鼠凝血纤溶因子的变化  

Changes of coagulation-fibrinolysis factors in rats with chronic hypoxia induced pulmonary hypertension

作　　者：李宜珊 康睿男 张舒婷 李杰娜 施熠炜[1] Li Yishan;Kang Ruinan;Zhang Shuting;Li Jiena;Shi Yiwei(Department of Respiratory and Critical Care Medicine, the First Hospital of Shanxi Medical University, Taiyuan 030001, China)

机构地区：[1]山西医科大学第一医院呼吸与危重症医学科,太原030001

出　　处：《国际呼吸杂志》2019年第6期408-412,共5页International Journal of Respiration

基　　金：山西省应用基础研究项目(201601D011110).

摘　　要：目的探讨凝血纤溶因子在慢性低氧所致肺动脉高压大鼠模型中的变化情况及意义。方法采用慢性常压低氧[氧浓度(10.0±0.1)%]肺动脉高压大鼠模型,将30只雄性SD大鼠随机分为正常对照组、低氧组(低氧3 d、7 d、14 d和21 d组),每组6只,用酶联免疫吸附试验(ELISA)法测定大鼠血浆血管性血友病因子(vWF)、蛋白C(PC)、组织型纤溶酶原激活物(t-PA)、纤溶酶原激活物抑制物1(PAI-1)水平,同时检测各组大鼠右心室收缩压(RVSP)和右心室肥厚指数(RVHI),观察各组大鼠血浆vWF、PC、t-PA、PAI-1水平及RVSP、RVHI值的变化,采用HE染色观察肺动脉病理学改变。结果(1)与对照组相比,低氧各组大鼠RVSP、RVHI均显著升高(P<0.01),低氧暴露3 d、7 d和21 d组肺动脉病理学改变明显;(2)与对照组相比,低氧各组大鼠血浆vWF、t-PA(除低氧7 d组)、PAI-1水平均显著升高(P<0.01),其中血浆vWF、PAI-1水平在低氧7 d组达高峰;低氧3 d、14 d、21 d组血浆PC水平均显著降低(P<0.01),低氧7 d组血浆PC水平显著升高(P<0.01);(3)慢性低氧性肺动脉高压大鼠血浆vWF、PAI-1水平与RVSP均呈正相关(P<0.01);(4)低氧各组与对照组相比大鼠血浆t-PA/PAI-1的比值水平均显著降低(P<0.01)。结论慢性低氧诱导的肺动脉高压大鼠血浆vWF、PC、t-PA、PAI-1明显变化,其中血浆vWF和PAI-1的水平可作为间接评价慢性低氧性肺动脉高压严重程度的指标。Objective To investigate the changes and significance of coagulation-fibrinolysis factors in rats with chronic hypoxia induced pulmonary hypertension. Methods Thirty male SD rats were randomly divided into five groups, and exposed to normoxia control or to normoxia hypoxia [(10.0±0.1)% oxygen] for 3, 7, 14 and 21 days to induced pulmonary hypertension.Von Willebrand factor (vWF), protein C (PC), tissue-plasminogen activator (t-PA) and plasminogen activator inhibitor1 (PAI-1) were analyzed by enzyme-linked immune sorbent assay (ELISA). Right ventricular systolic pressure (RVSP) was measured via catheterization, right ventricular hypertrophy index (RVHI) was calculated as the ratio of right ventricular mass to left ventricle plus septum mass.Pathological changes of the pulmonary arteries were observed by hematoxylin (HE) staining. Results(1)Compared with the control group, RVSP and RVHI increased significantly after hypoxic exposure on the third day, 7th day, 14th day and 21th day (P<0.01);The pathological changes of the pulmonary arteries were significant on the third day, 7th day and 21th day.(2)vWF, t-PA (except hypoxia group of day 7) and PAI-1 increased significantly after chronic hypoxia when compared with the control group (P<0.01). Especially, vWF and PAI-1 got peak in the hypoxia group on the 7th day (P<0.01). Compared with the control group, PC decreased obviously after hypoxic exposure on the third day, 14th day and 21th day (P<0.01), PC increased significantly after hypoxic exposure on the 7th day (P<0.01);(3)RVSP was positively correlated with the vWF and PAI-1 (P<0.01);(4)Compared with the control group, the ratio of t-PA to PAI-1 was significantly lower on the third day, 7th day, 14th day and 21th day (P<0.01). Conclusions Changes of coagulation-fibrinolysis factors in rats with chronic hypoxia induced pulmonary hypertension are obvious, vWF or PAI-1 can be used as an indicator to assess the severity of chronic hypoxia induced pulmonary hypertension.

关 键 词：高血压 肺性 纤溶酶原激活剂 缺氧 血压 

分 类 号：R544.1[医药卫生—心血管疾病]