两种小鼠肺癌模型的构建及Micro PET-CT观察  

Establishment of Two Kinds of Lung Cancer Models in Mouse and Evaluation by Micro PET-CT

作　　者：沈艳 王韵[2] 张汝 阮铮 毛建华 SHEN Yan;WANG Yun;ZHANG Ru;RUAN Zheng;MAO Jian-hua(Research Center for Experimental Medicine,Ruijin Hospital Affiliated toShanghai Jiao Tong University School of Medicine,Shanghai 200025,China;State Key Laboratory of Medical Genomics,Shanghai Instituteof Hematology,Ruijin Hospital Affiliated to Shanghai Jiao TongUniversity School of Medicine,Shanghai 200025,China;College of Veterinary Medicne,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]上海交通大学医学院附属瑞金医院实验医学研究中心,上海200025 [2]医学基因组学国家重点实验室,上海血液学研究所,上海交通大学医学院附属瑞金医院,上海200025 [3]南京农业大学,动物医学院,南京210095

出　　处：《实验动物与比较医学》2019年第1期39-45,共7页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金(81101721);上海市浦江人才计划项目(16PJ1406100);上海市自然科学基金(16ZR1421000)

摘　　要：目的比较尾静脉注射与肋间隙肺部原位注射人源非小细胞肺癌(NSCLC)细胞制备肺癌小鼠模型肿瘤形成情况的差异。方法使用NSCLC株NCI-H1975细胞,通过尾静脉注射以及经肋间隙肺部原位注射的方法制备NOD-SCID小鼠肺癌模型,于造模后15d,应用小动物PET-CT技术观察小鼠肺部肿瘤形成情况:解剖小鼠,检测肺部病理改变。结果造模15d后,经尾静脉注射NCI-H1975细胞的小鼠在肺部不能检测到肿瘤,而经肋间隙肺部原位注射NCI-H1975细胞的小鼠能够在肺部检测到明显的肿瘤形成。结论肋间隙肺部原位注射肺癌细胞制备的肺癌小鼠模型肺部肿瘤形成均一、稳定、可重复、高效,是较好制备原位肺癌模型的方法.Objective To compare the difference of two kinds of lung cancer models in mouse,which were established by injecting with the human nomsmall cell lung cancer(NSCLC)cells through tail vein or orthotopically inoculating in the lung using a microsyringe through the intercostals space.Methods The NCI-H1975 cells of NSCLC were injected into the NOD-SCID mouse via tail vein or intercostals space.After 15 days,mice were scanned by micro PET-CT to monitor the tumor growth and metastasis.Then mice were sacrificed and their lungs were dissected,fixed with phosphate-buffered neutral formalin and prepared for standard histological examination.Results Mice that were injected with the NCI-H1975 cells through tail vein for 15 days did not form tumor nodules on the lung.However,the mice that were orthotopically inoculated in the lung through intercostals space significantly had tumor nodules in their lungs.Conclusion A NOD-SCID mouse lung tumor model can be successfully established by transplanting NCI-H1975 cells into the lung through intercostals space.This model is simple,repeatable,and efficient.

关 键 词：原位肺癌 小鼠模型 小动物PET-CT 尾静脉注射 肋间隙注射 

分 类 号：Q95-33[生物学—动物学]