局部应用碘酊对兔游离皮瓣的影响  

Effect Evaluation on Rabbits Free Flaps with Locally Applying Iodine Tincture

作　　者：侯栋 程少华[1] 宋阳 宋鹏飞 王雷[1] 薛会朝[1] HOU Dong;CHENG Shao-hua;SONG Yang;SONG Peng-fei;WANG Leil;XUE Hui-chao(Department of General Surgery,The First Affiliated Hospital ofXinxiang Medical University,Xinxiang 453100,China)

机构地区：[1]新乡医学院第一附属医院普外三科

出　　处：《实验动物与比较医学》2019年第1期46-51,共6页Laboratory Animal and Comparative Medicine

基　　金：河南省科技厅科技攻关项目(172102310405)

摘　　要：目的构建兔皮下积液动物模型,探究质量分数2%碘酊对皮下积液及皮瓣愈合情况的影响。方法将32只新西兰兔随机分成A、B、C、D四组,A、B两组为对照组,C、D两组为处理组。处理组用2%碘酊处理游离皮瓣,对照组用生理盐水处理游离皮瓣。分别于术后3d处死A、C组,术后5d处死B、D组,检测体质量、白细胞、中性粒细胞百分比、皮下积液量,对游离皮瓣处组织进行组织病理学分析。结果处理组的皮下积液量少于对照组(P<0.05);月交原纤维增生占比及血管增生数量处理组均明显高于对照组(P<0.01)。体质量、白细胞计数及中性粒细胞百分比在四组间差异无统计学意义。结论2%碘酊能促进兔游离皮瓣处胶原纤维及血管增生,减少皮下积液,加速创面愈合。Objective To investigate the impact of local 2%iodine tincture application on healing wounding flap and reducing seroma formation following a model created in rabbits.Methods Thirtytwo New Zealand rabbits were randomly divided into groups A,B,C and D,group A and group B as control groups,group C and group D as treatment groups.The flaps of treatment groups were treated with 2%iodine tincture,and the control groups with normal saline,group A and group C were sacrificed on the third day after surgery,while group B and group D on the fifth day.We analyzed the results of weight,leukocyte count,neutrophil percentage,seroma volume of all groups and we evaluated tissue samples using histopathological examinations.Results Seroma formation was significantly decreased in groups C,D compared with groups A,B(P<0.05).The proportion of hyperplastic collagen fibrils and hyperplastic vessels number were both significantly higher in groups C,D than that of groups A,B(P<0.001).There is no significant difference among the four groups in terms of weight,leukocyte count and neutrophil percentage.Conclusion Two percent iodine tincture promoted angiogenesis and collagen synthesis of free flap,reduced seroma formation and accelerated wound healing of New Zealand rabbits.

关 键 词：乳腺癌 皮下积液 2%碘酊 改良根治术 

分 类 号：Q95-33[生物学—动物学]