SPF金黄仓鼠种群的建立及质量控制  

Establishment and Quality Control of SPF Golden Hamster Colonies

作　　者：张华琼 夏爽 吴燕茹 何凡 ZHANG Hua-qiong;XIA Shuang;WU Yan-ru;HE Fan(Chengdu Institute of Biological Products Co.Ltd.,Chengdu 610023,China)

机构地区：[1]成都生物制品研究所有限责任公司

出　　处：《实验动物与比较医学》2019年第1期56-60,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的为配合乙型脑炎减毒活疫苗世界卫生组织(WHO)预认证,建立高质量的SPF金黄仓鼠种群,方法通过剖宫产手术按照既定的配种方式建立SPF金黄仓鼠新种群,全面质量控制,定期抽样检测。结果建立了SPF金黄仓鼠核心群和生产群,19种病毒、6种寄生虫和17种细菌检测阴性。结论新建SPF金黄仓鼠种群质量符合WHOTRS980的要求。Objective To establish high-quality SPF golden hamster colonies in accordance w让h WHO pre-certification of live aMenuated Japanese Encephalitis Vaccine.Methods A new SPF golden hamster colony was established by caesarean based on the established mating method.The colonies were under general quality control with regular sampling tests.Results The foundation stocks and production stocks of SPF golden hamsters were established,which were negative in 19 virological items>6 parasitological items and 17 bacteriological items.Conclusion A new SPF golden hamster colony is successfully established,the quality meets the requirements of WHO TRS 980.

关 键 词：乙型脑炎活疫苗 预认证 SPF 金黄仓鼠 剖宫产 质量控制 

分 类 号：Q95-33[生物学—动物学]