贫血小板血清对人羊膜间充质干细胞增殖分化的影响  

Effect of serum from platelet-poor plasma on the proliferation and differentiation of human amnion mesenchymal stem cells

作　　者：刘晓玉 潘伯臣[1] 庞希宁[2] LIU Xiao-yu;PAN Bo-chen;PANG Xi-ning(Reproductive Medicine Center, Obstetrics and Gynecology Department, Shengjing Hospital, China Medical University, Shenyang 110122, China;Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang 110122, China)

机构地区：[1]中国医科大学附属盛京医院妇产科生殖医学中心,沈阳110022 [2]中国医科大学干细胞与再生医学研究室,沈阳110122

出　　处：《解剖科学进展》2019年第2期173-176,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然基金(201602848);盛京医院院内课题(ME89);沈阳双百工程项目(250039)

摘　　要：目的研究贫血小板血清(SPPP)对人羊膜间充质干细胞(hAMSCs)增殖分化的影响。方法利用酶消化法分离hAMSCs,制备SPPP培养hAMSCs,利用流式细胞术检测细胞表型,WST法绘制细胞生长曲线,诱导分化实验检测中胚层分化能力。结果 SPPP培养的hAMSCs表达CD73,CD90,CD105,不表达CD34,CD45,细胞倍增时间为72h,能够被诱导分化为骨,软骨,脂肪细胞。结论 SPPP作为hAMSCs的无动物源性成分培养基,为hAMSCs的细胞治疗及临床应用提供可能。Objective To evaluate the effect of serum from platelet-poor plasma(SPPP) on the proliferation and differentiation of human amnion mesenchymal stem cells(hAMSCs). Methods hAMSCs were isolated by enzymatic digestion and cultured in SPPP, then immunophenotype of hAMSCs was examined by flow cytometry.The cell growth curves were measured by WST assay. Results hAMSCs cultured in SPPP expressed CD73,CD90 and CD105 positively, but not express CD34 and Cd45, doubling time of hAMSCs was approximately 72h. The hAMSCs in SPPP medium were differentiated successfuly into osteoblasts, chondrocytes and adipocytes in appropriate induction medium. Conclusion SPPP has the potential to be used for the expansion of hAMSCs before clinical application.

关 键 词：人羊膜间充质干细胞 贫血小板血清 增殖 分化 

分 类 号：Q813[生物学—生物工程]