血栓心脉宁对血管性痴呆大鼠学习记忆功能与脑组织炎症因子表达的影响  

Effect of Xueshuan Xinmaining on learning and memory function and expression of inflammatory factors in brain tissue of vascular dementia mice

作　　者：付佳宇 刘宏雁[1] 于玮炜[2] Fu Jiayu;Liu Hongyan;Yu Weiwei(Department of Pharmacology, College of Pharmacy, Jilin University, Changchun 130021;Department of Emergency, Stomatological Hospital of Jilin University, Changchun 130021)

机构地区：[1]吉林大学药学院药理教研室,长春130021 [2]吉林大学口腔医院急诊科,长春130021

出　　处：《中药药理与临床》2019年第1期149-154,共6页Pharmacology and Clinics of Chinese Materia Medica

基　　金：吉林省中医药管理局中医药科技项目(2010-115)

摘　　要：目的:研究血栓心脉宁对血管性痴呆(VD)大鼠学习记忆能力及病理损伤的改善作用,并从炎症因子方面探讨其作用机制。方法:采用永久性结扎双侧颈总动脉法建立大鼠VD模型,实验分为对照组、模型组、甲磺酸双氢麦角毒碱片(喜得镇)0.54 mg/kg剂量组,血栓心脉宁0.55 g/kg、1.10 g/kg、2.20 g/kg剂量组。通过Morris水迷宫观察大鼠的学习记忆能力;采用苏木素-伊红(HE)染色考察大鼠海马及皮质的病理形态;采用免疫组化法检测大鼠脑组织海马CA1区胶质纤维酸性蛋白(GFAP)、肿瘤坏死因子-α(TNF-α)、核转录因子-κB(NF-κB p65)及白细胞介素-6(IL-6)的表达。结果:与模型组相比,血栓心脉宁0.55 g/kg、1.10 g/kg、2.20 g/kg剂量组对VD大鼠的学习记忆功能有明显的改善作用。海马CA1区GFAP、TNF-α、NF-κB p65及IL-6表达明显降低。血栓心脉宁1.10 g/kg、2.20 g/kg剂量组大鼠脑组织皮层区与海马区的病理损伤有明显改善作用。结论:血栓心脉宁可明显提高VD大鼠的学习记忆能力,改善受损海马及皮质组织;其保护作用机制可能与降低VD大鼠GFAP、TNF-α、NF-κB p65及IL-6表达有关。Objective: To study the effect of Xueshuan Xinmaining on learning memory and pathological damage in vascular dementia( VD) rat model,and to explore its mechanism related to inflammatory factors. Methods: Vascular dementia rat model was established by blocking bilateral common carotid arteries permanently. The rats were divided into the control group,the model group,0. 54 mg/kg Co-dergocrine Mesylate Tablets( Xidezhen) group,0. 55 g/kg,1. 10 g/kg and 2. 20 g/kg Xueshuan Xinmaining groups. The learning and memory abilities of rats were observed by Morris water maze test. The HE staining was used for the assessment of the histopathological changes in the hippocampus and cortex,and the expression levels of glial fibrillary acidic protein( GFAP),tumor necrosis factor-α( TNF-α),nuclear factor-κB( NF-κBp65) and interleukin-6( IL-6) in hippocampus CA1 region were detected by immunohistochemistry. Results: Compared with the model group,0. 55 g/kg,1. 10 g/kg and 2. 20 g/kg Xueshuan Xinmaining significantly improved the learning and memory functions of VD rats. The expression levels of GFAP,TNF-α,NF-κBp65 and IL-6 in hippocampus CA1 region were significantly decreased. The pathological damages of cerebral cortex and hippocampus in 1. 10 g/kg and 2. 20 g/kg Xueshuan Xinmaining groups were obviously improved. Conclusion: Xueshuan Xinmaining can improve the learning-memory abilities of VD rats and alleviate the damage in hippocampus and cortex. The protective mechanism may be related to down-regulating expressions of GFAP,TNF-α,NF-κBp65 and IL-6 in hippocampus of vascular dementia rats.

关 键 词：血栓心脉宁 血管性痴呆 神经胶质酸性蛋白 肿瘤坏死因子-α 核转录因子-ΚB 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]