穿心莲内酯及其衍生物抗肠道病毒效果评价  

Evaluation of Anti-enterovirus Effect of Andrographolide and Its Derivatives

作　　者：陈田 毛夷冬 马年 沈梦欣 曾君南 蒋春红 刘媛媛[1] 刘东瀛[1] 熊海蓉[1] 杨占秋[1] Chen Tian;Mao Yidong;Ma Nian;Shen Mengxin;Zeng Junnan;Jiang Chunhong;Liu Yuanyuan;Liu Dongying;Xiong Hairong;Yang Zhanqiu(Institute of Medical Virology/State Key Laboratory of Virology, Wuhan University, Wuhan 430071 , China;Jiangxi Qingfeng Pharmaceutical Co. Ltd.)

机构地区：[1]武汉大学医学病毒学研究所/病毒学国家重点实验室,武汉430071 [2]江西青峰制药有限公司

出　　处：《中国药师》2019年第4期635-639,共5页China Pharmacist

摘　　要：目的:评价穿心莲内酯及其衍生物(XYP-1、XYP-2及XYP-3)抑制柯萨奇病毒B3型(CVB3)感染MA104细胞的效果。方法:采用MTT法检测3种中药注射液对MA-104细胞的细胞毒性,并通过感染阻断、直接杀伤、增殖抑制3种不同作用方式评价药物对CVB3感染细胞的体外抗病毒效果。结果:穿心莲内酯及其衍生物在阻断病毒感染细胞作用中,对CVB3的治疗指数(TI)分别为15.29,6.76,1.86;在阻止病毒增殖抑制作用中,对CVB3的TI分别为20.00,50.00,1.67;在直接杀伤病毒作用中,对CVB3的TI分别为7.65,25.00,1.83。结论:穿心莲内酯及其衍生物在不同作用方式下,对CVB3病毒具有不同程度的抑制效果,XYP-1主要阻断病毒的感染并抑制病毒增殖,XYP-2则能抑制病毒增殖并对病毒有杀伤作用,而XYP-3抗病毒效果较差。Objective: To evaluate the effects of andrographolide and its derivatives( XYP-1,XYP-2 and XYP-3 injections) on the inhibition of coxsackievirus B3( CVB3) in MA-104 cells. Methods: The MTT assay was used to detect the cytotoxicity,and the in vitro efficacy of injections on CVB3 infected cells were evaluated by using prophylactic activity,virucidal activity and anti-proliferation activity as the indices. Results: The antiviral therapeutic index( TI) of andrographolide and its derivatives for CVB3 respectively was15. 29,6. 76 and 1. 86 in prophylactic activity,in the anti-proliferation activity,the TI for CVB3 respectively was 20. 00,50. 00 and1. 67,and in the virucidal activity,the TI for CVB3 was 7. 65,25. 00 and 1. 83,respectively. Conclusion: Andrographolide and its derivatives have different inhibitory effects on CVB3 virus with various action modes. XYP-1 takes advantage in prophylactic activity and anti-proliferation activity,XYP-2 mainly has antiviral effects in anti-proliferation activity and virucidal activity,while XYP-3 shows poorer antiviral effects.

关 键 词：穿心莲内酯 柯萨奇病毒 体外药效 抗病毒治疗指数 

分 类 号：R965.1[医药卫生—药理学]