犬细小病毒2型变异株对高母源抗体犬的致病性  

The pathogenicity of canine parvovirus-2 variants in puppies with highly maternal antibody

作　　者：甘军纪[1] 田晓彦[1] 高巍[1] 刘秀梵[1] GAN Jun-ji;TAN Xiao-yan;GAO Wei;LIU Xiu-fan(Jiangsu Co-innovation Center for Im portantAnimal Infectious Diseases and Zoonoses , College of Veterinary Medicine ,Yangzhou University ,Yangzhou ,Jiangsu 225009,China)

机构地区：[1]扬州大学兽医学院江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医学报》2019年第3期408-413,共6页Chinese Journal of Veterinary Science

基　　金：江苏省动物重要疫病与人兽共患病防控协同创新中心;江苏高校优势学科建设工程资助项目

摘　　要：为了探究CPV-2变异株的致病性,用CPV-2a和CPV-2b野毒株,分别攻击犬细小病毒(CPV-2)高母源抗体(MDA)幼犬,根据犬临床症状,组织病理学,粪便排毒和血清抗体应答等指标,评价高母源抗体(HI滴度≥1∶160)对不同CPV病毒变异株的保护作用。结果表明, CPV-2a和CPV-2b攻毒后引起明显临床症状,攻毒后3～6 d粪便中CPV排毒。感染犬的组织样品经PCR检测表明,CPV-2a/2b病毒广泛分布。组织病理学分析表明,CPV-2a/2b感染引起肠道黏膜出血。研究表明,CPV-2高母源抗体对CPV-2a/2b变异株攻击不能提供有效保护,有必要开发CPV变异株的新型疫苗。To investigate the pathogenicity of these CPV-2 variants,the puppies with high-titer maternally derived antibody(MDA) to CPV were challenged with CPV variants CPV-2 a and CPV-2 b,respectively.Clinical signs,histopathological changes,virus shedding and antibody responses were detected to evaluate the protection potency of the high-titer MDA(haemagglutination inhibiting(HI) titer≥ 1∶160)against different CPV variants.In comparison with the puppies challenged with CPV-2,the puppies challenged with CPV-2 a and CPV-2 b showed obvious clinical signs and shed viruses in feces from day 3 to day 6 post challenge.Moreover,CPV-2 a and CPV-2 b viruses could be detected by PCR with wide distribution in the tissues.The histopathological analysis further showed that the intestine mucosal hemorrhage could be found in puppies infected with CPV-2 a and CPV-2 b.This finding demonstrate that high-titer MDA to CPV-2 could not effectively protect puppies from CPV-2 a/2 b infection,calling for novel CPV vaccines against CPV-2 variants.

关 键 词：犬细小病毒 变异株 母源抗体 致病性 

分 类 号：S852.65[农业科学—基础兽医学]