鸭源鸡杆菌gtxA突变株构建及其生物特性分析  

Construction and characterization of gtxA gene mutant strain of Gallibacterium anatis

作　　者：王继洋[1] 彭志锋 刘盼盼 王坤芃 王川庆[1] 陈陆[1] 赵军[1] 杨霞[1] WANG Ji-yang;PENG Zhi-feng;LIU Pan-pan;WANG Kun-peng;WANG Chuan-qing;CHENLu;ZHAO Jun;YANG Xia(College of Animal Medicine Engineering , Henan Agricul tural University , Zhengzhou 450046 , China;College of Animal Medicine , Henan College of Animal Economics,Zhengzhou 450046 ,China)

机构地区：[1]河南农业大学牧医工程学院,河南郑州450046 [2]河南牧业经济学院动物医学学院,河南郑州450046

出　　处：《中国兽医学报》2019年第3期414-420,共7页Chinese Journal of Veterinary Science

基　　金：河南省自然科学基金资助项目(162300410153);河南省高校科技创新团队与支持计划资助项目(14IRTSHN015);河南省科技攻关项目(172102110046)

摘　　要：为了了解毒素GtxA对鸭源鸡杆菌(Gallibacterium anatis,G.anatis)生物学特性及致病性的影响,利用自然转化和正向筛选法构建了1株G.anatis gtxA缺失突变株,初步探究突变株的生物学特性。与亲本菌株比较,突变株溶血活性消失,菌落形态及生长速率并未出现显著改变,其对小鼠的致病力下降。外毒素GtxA参与G.anatis致病过程并扮演一定角色;G.anatis gtxA基因突变株的构建及其生物学特性探究为更好的了解其致病机理和研发出更为高效安全疫苗建立了理论基础。To determine the biological role of toxin GtxA of Gallibacterium anatis,a Gallibacterium anatis gtxA deleted mutant was constructed by natural transformation,The gtxA deleted mutant was confirmed by PCR,SDS-PAGE,Western blot and heredity seability assays.The biological characterics of the mutant strain was investigated.The results demonstrated that the mutant lose hemolytic activity and possess the same growth rate as its parental strain,but it attenuated in mice.These results indicated that GtxA was an important virulence factor of G.anatis.This study have further illumination on the pathogenicity of G.anatis and it will facilitate the development of a reasonable live attenuated vaccines of G.anatis.

关 键 词：G.anatis gtxA基因 突变株 致病性 

分 类 号：S852.61[农业科学—基础兽医学]