黑皮质素4型受体在绵羊下丘脑区域的定位  

Location of the melanocortin 4 receptor in sheep hypothalamus

作　　者：郭凤英[1] 潘登[2] 范奎奎[2] 李强[2] 杨文雅 钱英红 杜晨光[1,2] GUO Feng-Ying;PAN Deng;FAN Kui-Kui;LI Qiang;YANG Wen-Ya;QIAN Ying-Hong;DU Chen-guang(Inner Mongolia Agricultural University Vocational and Technical College ,Baotou,Inner Mongolia 014109 ,China;College of Veterinary ,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学职业技术学院,内蒙古包头014109 [2]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2019年第3期507-511,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31660701);内蒙古自然科学基金资助项目(2018MS03046);内蒙古高等学校青年科技英才计划项目(NJYT-12B12)

摘　　要：黑皮质素4型受体(melanocortin 4 receptor,MC4R)作为下丘脑参与能量调控过程中的关键性受体,在动物能量代谢调节中被广泛关注。目前,关于该受体在绵羊下丘脑中分布区域的相关报道较少。针对试验过程中MC4R免疫荧光结果不稳定,分布区域不明显的特点,本研究在改进现有MC4R免疫荧光试验方法基础上,对MC4R在绵羊下丘脑的分布区域进行了观察。结果显示,分别经过4%多聚甲醛长时间固定(6 d),Triton X-100(pH≈8.68)长时间通透(2 d),以及3%过氧化氢和0.2 mol/L甘氨酸+0.3%SDS+20%甲醇的预处理后,可以获得稳定且饱满的MC4R免疫荧光结果。MC4R主要分布在绵羊下丘脑的室旁核(arcuate nucleus,ARC)区域,但在弓状核区域并无类似分布。Melancholic 4 receptor(MC4 R) is a key receptor involved in energy regulation in the hypothalamus and is widely concerned in bodily energy metabolism.At present,the distribution of this receptor in sheep hypothalamus is unclear.Owing to MC4 R immunofluorescences were unstable and there is not sufficient evidence to prove the distribution area.Here,based on the improvement of the MC4 R immunofluorescence assay,we identified the distribution of MC4 R in the hypothalamus of sheep,through using,long-time external fixation(6 days),TritonX-100(pH≈8.68) for 4 days,and progressing the pretreatment with 3% hydrogen peroxide and 0.2 mol/L glycine + 0.3% SDS + 20% methanol.MC4 R immunofluorescence results can be stably obtained,and is mainly distributed in the paraventricular nucleus of hypothalamus(PVH) of sheep and does not show a concentrated distribution in the arcuate nucleus(ARC) neucleus.

关 键 词：黑皮质素4型受体 免疫荧光 绵羊 下丘脑 

分 类 号：S852.21[农业科学—基础兽医学]