头穴丛刺对阿尔茨海默病大鼠海马区ChAT、AchE表达的影响  

Effects of cluster needling at the scalp points on the expression of ChAT and AchE of hippocampus in rats with Alzheimer's disease

作　　者：李虹霖[1] 高伟 夏昆鹏[1] 孙琦月 佟晓薇 栾凯迪 朱红奇 齐慧敏 朱斌 徐飞[3] LI Hong-lin;GAO Wei;XIA Kun-peng;SUN Qi-yue;TONG Xiao-wei;LUAN Kai-di;ZHU Hong-qi;QI Hui-min;ZHU Bin;XU Fei(the Second Affiliated Hospital of Heilongjiang University of CM, Harbin 150001, China;Heilongjiang University of CM;Heilongjiang Provincial Hospital, Harbin 150036)

机构地区：[1]黑龙江中医药大学附属第二医院,哈尔滨150001 [2]黑龙江中医药大学 [3]黑龙江省医院,哈尔滨150036

出　　处：《中国针灸》2019年第4期403-408,共6页Chinese Acupuncture & Moxibustion

基　　金：黑龙江省自然科学基金青年基金项目:QC2017116;哈尔滨市科技局科技创新人才项目:2016RAQXJ164;黑龙江中医药大学附属第二医院科研项目基金:YZ-201701;黑龙江中医药大学研究生创新科研项目:yjscx2017045

摘　　要：目的:探讨头穴丛刺对阿尔茨海默病(Alzheimer’s disease,AD)大鼠海马区乙酰胆碱转移酶(ChAT)、乙酰胆碱酯酶(AchE)表达的影响。方法:将60只Wistar大鼠随机分为假手术组、模型组、药物组、丛刺组,每组15只。模型组、药物组、丛刺组采用Aβ1-42向大鼠双侧海马CA1区定向注射诱导阿尔茨海默病(AD)模型,假手术组给予双侧海马区注射等量蒸馏水。药物组给予多奈呱齐(安理申)灌胃作为基础治疗,每日1次,共治疗4周;丛刺组在药物组的基础上加头穴丛刺治疗,每日1次,每周休息1d,共干预4周;假手术组、模型组给予常规喂养。干预后利用Morris水迷宫检测大鼠学习记忆能力;HE染色观察大鼠海马大体结构的变化;免疫组化观察大鼠海马区Ch AT、Ach E阳性表达情况。结果:与假手术组比较,模型组大鼠的逃避潜伏期延长(P<0.01),第二象限百分比和跨越平台次数减少(均P<0.01);与模型组比较,丛刺组、药物组大鼠的逃避潜伏期缩短(P<0.05,P<0.01),第二象限百分比和跨越平台次数增加(P<0.05,P<0.01);与药物组比较,丛刺组大鼠的逃避潜伏期缩短(P<0.05),第二象限百分比和跨越平台次数增加(均P<0.05)。与假手术组比较,模型组ChAT表达下降(P<0.01),Ach E表达上升(P<0.01);与模型组相比,药物组Ch AT差异无统计学意义(P>0.05),AchE表达下降(P<0.05),丛刺组海马区ChAT表达增加(P<0.05),AchE表达下降(P<0.01);与药物组相比,丛刺组ChAT表达上升(P<0.05),AchE表达下降(P<0.05)。结论:头穴丛刺辅助治疗AD的机制之一可能是上调海马区ChAT活性及下调AchE活性,头穴丛刺联合安理申疗效更佳。Objective To explore the effects of cluster needling at the scalp points on the expression of choline acetyl transferase(ChAT) and choline cholinesterase(AchE). Methods A total of 60 Wistar rats were randomized into a sham-operation group, a model group, a medication group and a cluster needling group, 15 rats in each one. In the model group, the medication group and the cluster needling group, the models of Alzheimer’s disease(AD) were established by the orienteering injection with Aβ1-42 in the bilateral hippocampal CA1 in the rats. In the sham-operation group, the distilled water was injected in bilateral hippocampus of rats. In the medication group, the lavage with aricept was adopted for the basic treatment, once a day, for 4 weeks consecutively. In the cluster needling group, on the base of the treatment as the medication group, the cluster needling at the scalp points was adopted, once a day, 6 times a week, for 4 weeks totally. In the sham-operation group and the model group, the normal feeding was provided. After intervention, the learning and memory ability was measured with Morris water maze in the rats of each group. The changes in the hippocampal gross structure were observed with HE staining. The changes in the positive expressions of hippocampal ChAT and AchE were determined with the immunohistochemical method. Results Compared with the sham-operation group, the escape latency was prolonged and the percentage of the second quadrant and the frequency of platform leaping were reduced in the rats of the model group(all P<0.01). Compared with the model group, the escape latency was shortened and the percentage of the second quadrant and the frequency of platform leaping were increased in the rats of the cluster needling group and the medication group(P<0.05,P<0.01). Compared with the medication group, the escape latency was shortened and the percentage of the second quadrant and the frequency of platform leaping were increased in the rats of the cluster needling group(all P<0.05). Compared with the

关 键 词：阿尔茨海默病 老年痴呆 头穴丛刺 海马 乙酰胆碱酯酶 乙酰胆碱转移酶 

分 类 号：R245.9[医药卫生—针灸推拿学]