参苓白术散对感染鼠伤寒沙门菌小鼠的免疫调节作用  

Immunomodulatory effects of Shenlin Baizhu San on mice infected with Salmonella typhimurium

作　　者：高玲玲 单颖 GAO Ling-ling;SHAN Ying(Jinzhou Medical University, Jinzhou, Liaoning 121000, China)

机构地区：[1]锦州医科大学,辽宁锦州121000 [2]北部战区总医院,辽宁沈阳110000

出　　处：《现代预防医学》2019年第8期1450-1455,共6页Modern Preventive Medicine

基　　金：辽宁省自然科学基金指导计划项目(201602276)

摘　　要：目的探讨参苓白术散对感染鼠伤寒沙门菌小鼠的抑菌作用及免疫调节机制。方法用鼠伤寒沙门菌标准菌株(STM,50115)制备STM小鼠模型,造模后给予小鼠参苓白术散(1 g/ml)或庆大霉素(2 mg/ml)连续灌胃10 d,观察小鼠盲肠病理改变,检测盲肠中TNF-α、IL-1β、IL-6、TLR4、MyD88、NF-κB mRNA水平及TLR4、MyD88、NF-κB蛋白含量,测定淋巴结中CD4^+T、CD8^+T及CD4^+IFN-γ和CD8^+IFN-γ细胞比例。结果与对照组比较,STM模型组TNF-α、IL-1β、IL-6及TLR4、MyD88、NF-κB mRNA水平升高(P<0.01),参苓白术散组和庆大霉素组较比STM模型组呈下降趋势(P<0.05);STM模型组TLR4、MyD88、NF-κB蛋白含量高于对照组(P<0.01),参苓白术散组和庆大霉素组低于STM模型组(P<0.05);STM模型组CD4^+T、CD8^+T及CD4^+IFN-γ和CD8^+IFN-γ细胞比例均高于对照组(P<0.05),参苓白术散组高于STM模型组(P<0.05),庆大霉素组较STM模型组有下降趋势(P>0.05)。结论参苓白术散对STM感染具有一定治疗效果,治疗机制可能与下调TLR4-MyD88-NF-κB信号诱导的炎性细胞因子分泌及上调CD4^+T、CD8^+T比例及功能有关。Objective To investigate the antibacterial effect and immunoregulatory mechanism of Shenqi Baizhu San on mice infected with Salmonella typhimurium(STM). Methods The models of Salmonella typhimurium were constructed using standard strains(STM, 50115). After the models were established, the mice of the cecal were observed. The m RNA expression of inflammatory cytokines of TNF-α, IL-1β, IL-6, TLR4, MyD88, and NF-κB were detected. Then we analyzed the protein expression of TLR4, My D88, and NF-κB, and the proportion of CD4^+T, CD8^+T, CD4^+IFN-γ and CD8^+IFN-γ cells in the lymph nodes. Results Compared with control group, the levels of TNF-α, IL-1β, IL-6, TLR4, MyD88, and NF-κB mRNA were higher in STM model group(P <0.01), while those cytokines in Shenlin Baizhu San group and gentamicin group were lower than in STM model group(P<0.05). The protein concentration of TLR4, MyD88 and NF-κB in STM model group were higher than that in control group(P<0.01), and lower in Shenlin Baizhu San group and gentamicin group than in STM model group(P<0.05). The proportions of CD4^+T, CD8^+T, CD4^+IFN-γ and CD8^+IFN-γ cells in STM model group were higher than those in control group(P<0.05), and those cells in Shenlin Baizhu San group were higher than that in STM model group(P<0.05). Compared with STM model group, the proportions of CD4^+T, CD8^+T, CD4^+IFN-γ and CD8^+IFN-γ cells in gentamicin group had a downward trend(P >0.05). Conclusion Shenqi Baizhu San has a therapeutic effect on STM infection, and its therapeutic mechanism may be related to the down-regulation of the secretion of various inflammatory cytokines induced by TLR4-MyD88-NF-κB signaling pathway, and the up-regulation of percentage and function of CD4^+T and CD8^+T cells.

关 键 词：参苓白术散 鼠伤寒沙门菌 TOLL样受体 CD4^+T CD8^+T 

分 类 号：R181.24[医药卫生—流行病学]