高效液相色谱法同时测定大气PM_(2.5)中15种欧盟优控多环芳烃  

Simultaneous determination of 15 EU controlled polycyclic aromatic hydrocarbons in atmospheric PM_(2.5) by high performance liquid chromatography

作　　者：王钟[1] 邢燕[1] 何漪[1] 王敏[1] 王勤[1] WANG Zhong;XING Yan;HE Yi;WANG Min;WANG Qin(Zibo Center for Diseases Control and Prevention, Zibo, Shandong 255026, China)

机构地区：[1]淄博市疾病预防控制中心,山东淄博255026

出　　处：《现代预防医学》2019年第8期1464-1467,共4页Modern Preventive Medicine

摘　　要：目的采用高效液相色谱-荧光检测技术,建立大气细颗粒物(PM_(2.5))中15种欧盟优控多环芳烃(EU-PAHs)的同时测定方法。方法利用超声波辅助乙腈提取PM_(2.5)样品中的EU-PAHs,经0.45μm滤膜过滤,用Waters PAH色谱柱分离,用乙腈-水作流动相梯度洗脱,荧光检测器检测,峰面积标准曲线法定量。结果方法线性范围为0.30～84ng/m^3,相关系数均大于0.999,方法精密度为4.6%～10.2%,回收率83.2%～95.6%。结论该方法灵敏度高,准确度高,线性范围宽,适用于大气PM_(2.5)中15种欧盟优控多环芳烃的同时检测。Objective To establish a simultaneous determination method of 15 EU controlled polycyclic aromatic hydrocarbons(EU-PAHs) in fine particulate matter(PM2.5) by high performance liquid chromatography with fluorescence detector was developed. Methods The EU-PAHs were ultrasound extraction with acetonitrile, then filtered with 0.45μm millipore filter. The separation was accomplished on a Waters PAH column, under gradient elution with the mobile phase composed of acetonitrile and water. Fluorescence detector was used for detection and the quantification was based on peak area standard curves.Results The linear ranges of the 15 EU-PAHs were 0.30-84 ng/m3 with the correlation coefficients greater than 0.999. The relative standard deviations of the method were 4.6%-10.2%, and the recovery rates were 83.2%-95.6%. Conclusion The method is sensitive, accurate, with wide linear ranges and it is suitable for the simultaneous detection of 15 EU-PAHs in PM2.5.

关 键 词：大气PM2.5 高效液相色谱法 欧盟优控多环芳烃 

分 类 号：R122[医药卫生—环境卫生学]