武汉城市湖泊抗生素及抗性基因的污染特征研究  

Pollution Characteristics of Antibiotics and Antibiotic Resistance Genes in Urban Lakes of Wuhan

作　　者：肖鑫鑫 吴亦潇 丁惠君[2] 万亮 杨文锋 张维昊 XIAO Xinxin;WU Yixiao;DING Huijun;WAN Liang;YANG Wenfeng;ZHANG Weihao(School of Resource and Environmental Science. Hubei Key Laboratory for Biomass-Resource Chemistry and Environmental Biotechnology, Wuhan University, Wuhan 430079, China;Key Laboratory of Water Resources and Environment of Poyang Lake of Jiangxi Province, Jiangxi Province Institute of Water Science, Nanchang 330029, China)

机构地区：[1]武汉大学资源与环境科学学院生物质资源化学与环境生物技术湖北省重点实验室,湖北武汉430079 [2]江西省水利科学研究院江西省鄱阳湖水资源与环境重点实验室,江西南昌330029

出　　处：《环境科学与技术》2019年第3期9-16,共8页Environmental Science & Technology

基　　金：国家自然科学基金项目(51869006);江西省自然科学基金项目(20171BAB216050);江西省水利科技项目(KT201607;KT201702);武汉大学大型仪器设备开放补贴资助项目(LF20181039)

摘　　要：为研究武汉城市湖泊抗生素及抗生素抗性基因的污染水平与分布特征,文章采用超高效液相色谱-质谱法和荧光定量PCR法对南湖、沙湖和东湖水体及底泥中12种抗生素、9种ARGs及Ⅰ类整合子intI1进行定性和定量分析。结果表明,湖泊水体及底泥中均以喹诺酮类抗生素污染为主,浓度范围分别为51.43～105.62 ng/L和9.01～12.93 ng/g。10种目标基因中,tetG、tetM、sul1、sul2、qnrD及intI1的检出率均为100%。磺胺类抗性基因sul1的相对丰度最高(2.39×10^(-2)～3.99×10^(-1)),属于优势抗性基因。intI1含量与4种抗性基因含量(tetG、tetM、sul1及qnrD)、ARGs总量之间均存在显著正相关关系(P<0.05),说明intI1是ARGs在城市湖泊环境中进行水平基因转移的重要媒介。冗余分析表明四环素类、喹诺酮类抗生素污染和intI1含量是影响湖泊水体、底泥中ARGs丰度及分布的重要因素。In order to investigate the pollution status and distribution characteristics of antibiotics and antibiotic resistance genes(ARGs) in urban lakes of Wuhan, high performance liquid chromatography-mass spectrometry and quantitative PCR were applied to analyze the occurrence and quantities of twelve antibiotics, nine ARGs and class I integron gene intI1 in water and sediment samples of South Lake, Sand Lake and East Lake. The results showed that quinolones were the main contaminants in all samples. The total concentration of quinolones ranged from 51.43 to 105.62 ng/L in water and from 9.01 to 12.93 ng/g in sediments, respectively. Among the ten tested genes, tetG, tetM, sul1, sul2, qnrD as well as intI1 had 100% detection frequency. Sul1 had the highest relative abundance(2.39×10-2~3.99×10-1), and was the predominant resistance gene. There was a significant positive correlation between the relative abundance of intI1 and four resistance genes(tetG, tetM, sul1 and qnrD) as well as total ARGs(P<0.05), indicating that intI1 was a significant media for horizontal gene transfer of ARGs in urban lakes. And redundancy analysis showed that tetracyclines, quinolones and intI1 may play a very important role in the abundance and distribution of ARGs in urban lakes of Wuhan.

关 键 词：城市湖泊 抗生素 抗生素抗性基因 荧光定量PCR 

分 类 号：X524[环境科学与工程—环境工程]