LDL与Raw264.7细胞中肾素-血管紧张素系统表达  

LDL activates renin-Angiotensin system in Raw264.7 cells

作　　者：刘南 章程 武舒佳 武军驻[1] Liu Nan;Zhang Cheng;Wu Shujia;Wu Junzhu(Dept of Biochemistry and Molecular Biology, Wuhan University School of Basic Medical Sciences, Wuhan 430071,China)

机构地区：[1]武汉大学基础医学院生物化学及分子生物学系,湖北武汉430071

出　　处：《医药前沿》2019年第8期26-28,共3页Journal of Frontiers of Medicine

基　　金：国家自然科学基金资助项目(81170273).

摘　　要：目的:探究巨噬细胞Raw264.7氧化低密度脂蛋白(LDL)过程中肾素-血管紧张素系统(RAS)的变化。方法:培养RAW264.7细胞,LDL刺激后,用ACE活性测定试剂盒检测细胞内ACE活性的变化,用AngU试剂盒测定AngD的表达;Real-TimePCR检测血管紧张素转移酶(ACE)mRNA的表达;利用Real-TimePCR及WesternBlot检测血管紧张素U2型受体(AGTR2、AT2R)的表达。结果:LDL刺激Raw264.7细胞后,细胞内ACE的活性.AngH的表达、ACEmRNA水平、AT2RmRNA和蛋白质的表达都升高(P<0.05)。结论:L叽激活了肾素-血管紧张素系统。Objective To explore the changes of renin-angiotensin system (RAS) in the process of low-density lipoprotein (LDL) oxidation in Raw264.7 macrophage cells.Methods Raw264.7 cells were cultured and stimulated with LDL and then ACE activity was determined with Mouse ACE ELISA Kit, Anglfs expression was determined with Mouse Angll ELISA Kit;Angiotensin I-converting enzyme (ACE) was detected by Real-Time PCR, angiotensin II type 2 receptor (AGTR2, AT2R) was detected by Real-Time PCR and Western Blot Results After LDL stimulated Raw264.7 cells, the activity of ACE, AnglFs expression, ACE mRNA level, AT2R's expression were increased (P<0.05).Conclusion LDL activates renin-angiotensin system in Raw264.7 cells.

关 键 词：低密度脂蛋白 肾素-血管紧张素系统 血管紧张素转移酶 

分 类 号：R541.4[医药卫生—心血管疾病]