防风多糖对过敏性鼻炎大鼠血清及鼻腔灌洗液细胞因子的影响  

Effect of Saposhnikovia Divaricata Polysaccharide on Cytnkines in Serum and Nasal Lavage Fluid of Rats with Allergic Rhinitis

作　　者：马志跃[1] 李彬[1] 冯勇[1] 余晓旭[1] 樊建刚[1] 何刚[1] Ma Zhiyue;Li Bin;Feng Yong;Yu Xiaoxu;Fan Jiangang;He Gang(Sichuan Academy of Medical Sciences,Sichuan People's Hospital Chengdu 610072, China)

机构地区：[1]四川省人民医学科学院·四川省人民医院耳鼻咽喉头颈外科,四川成都610072

出　　处：《医药前沿》2019年第8期41-43,共3页Journal of Frontiers of Medicine

基　　金：四川省成都市科技局课题(2015-HM01-00172-SF).

摘　　要：目的:考察防风多糖对过敏性鼻炎(AR)大鼠细胞因子的影响。方法:32只健康SD大鼠随机分空白组(A)、模型组(B)、防风多糖低剂量组(C)、防风多糖高剂量组(D),除A组外其余3组建立大鼠AR模型。每组于致敏第15d后开始给药,C、D组分别灌胃300、600mg/kg防风多糖治疗,A、B组灌胃等量0.9%生理盐水,连续14d,处死,采样待检。ELISA检测血清IgE、IL-4、INP-y及鼻腔灌洗液IgE、IL-4、HA含量。结果:C、D组较B组显著降低血清及鼻腔灌洗液IgE、IL-4含量、显著升高血清IFN-y含量,降低鼻腔灌洗液HA含量(P<0.05);D组较C组可显著改善血清及鼻腔灌洗液各扌旨标含量(P<0.01)。结论:防风多糖可调节AR细胞因子的生成和释放,对AR有治疗作用。Objective To investigate the effect of Saposhnikovia Divaricata polysaccharide on cytokines in rats with allergic rhinitis (AR). Methods Thirty-two healthy SD rats were randomly divided into blank group (A), model group (B), low-dose polysaccharide group (C), and high-dose polysaccharide group (D), rat AR models were established in the remaining 3 groups except group A. Each group was started after the 15th day of sensitization. Groups C and D were treated with 300,600 mg/kg of saposhnikovia divaricata polysaccharide, respectively Groups A and B were intragastrically administered with 0.9% normal saline for 14 days and the rats were sacrificed and samples were taken for examination. The levels of serum IgE, IL-4, INF-y and nasal lavage fluid IgE, IL-4 and HA were detected by ELISA. Results The levels of IgE, IL-4 in serum and nasal lavage fluid were significantly lower in group C and D than in group B, the content of serum IFN-y was significantly increased and HA in nasal lavage fluid was decreased (Pv0.05). The content of serum and nasal lavage fluid in group D was significantly improved compared with group C (PvO.Ol). Conclusions The saposhnikovia divaricata polysaccharide can regulate the production and release of AR cytokines and has a therapeutic effect on AR.

关 键 词：过敏性鼻炎 防风多糖 免疫因子 大鼠 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]