新疆地区牛种布鲁菌流行株的分子分型鉴定  

Molecular typing of Brucella abortus strains in Xinjiang

作　　者：叶锋[1] 马晓菁[1] 刘丽娅[1] 谢彩云 谷文喜[1] 钟旗[1] 马俊杰[1] 易新萍[1] Ye Feng;Ma Xiaojing;Liu Liya;Xie Caiyun;Gu Wenxi;Zhong Qi;Ma Junjie;Yi Xinping

机构地区：[1]新疆畜牧科学院兽医研究所,乌鲁木齐830011

出　　处：《中华地方病学杂志》2019年第4期265-268,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金(31660721).

摘　　要：目的鉴定新疆地区分离的牛种布鲁菌流行株分子分型,确定多位点可变数目串联重复序列分析(MLVA)法在布鲁菌种型水平上的鉴定和鉴别能力。方法应用优化的布鲁菌特异性PCR(AMOS-PCR)法对2010-2015年新疆地区分离的牛种布鲁菌流行株(n=7)进行鉴定。同时采用MLVA-16法对流行株进行种属和分子分型,将分型结果与http://mlva.u-psud.fr提供的布鲁菌MLVA基因型数据库进行比较,结合Bionumerics6.6软件进行聚类分析。结果AMOS-PCR和MLVA-16法对布鲁菌种属鉴定结果一致,均为牛种。MLVA-16法进一步分子分型鉴定结果均为牛种3型,与国内存在的牛种布鲁菌株的种型基本一致。结论新疆地区分离的牛种布鲁菌流行株分子分型鉴定为牛种3型。MLVA方法可以在种的水平鉴定布鲁菌,对布鲁菌生物型和株间差异有很高的鉴别力,可为鉴别布鲁菌病疫情流行株的溯源进化关系提供依据。Objective To identify molecular typing of Brucella abortus isolates in Xinjiang,and determine the identification ability of multiple locus variable-number tandem repeat analysis(MLVA).Methods The optimized Brucella AMOS-PCR was used for identification of Brucella(n= 7)genus and species in Xinjiang from 2010-2015,and MLVA-16 was used to further identify the isolates.Results were compared with the data of the Brucella standard strain provided by the http://mlva.u-psud.fr database.Cluster analysis was carried out with Bionumerics 6.6.Results The results of AMOS-PCR and MLVA-16 were identical,all were Brucella abortus.Further classification results of the MLVA-16 showed that the strain in Xinjiang was type 3 of Brucella abortus,which was basically the same as that of the domestic Brucella.Conclusions The molecular typing of isolates separated in Xinjiang is type 3 of Brucella abortus.MLVA can identify Brucella at the level of species,and highly sensitive to Brucella biotype and isolates differences,which provides a basis for the traceability and evolution of brucellosis epidemic strains.

关 键 词：布鲁杆菌属 生物学鉴定 细菌分型技术 

分 类 号：R516.7[医药卫生—内科学]