转基因HaBADH马铃薯无性一代耐盐性及农艺性状分析  

Analysis of Salt Tolerance and Agronomic Traits of Transgenic HaBADH Potato Asexual Generation

作　　者：吴英英 张丽[1,2] 巩檑[1,2] 甘晓燕[1,2] 聂峰杰[1,2] 陈虞超[1,2] 石磊[1,2] 张海雯 宋玉霞[1,2] Wu Yingying;Zhang Li;Gong Lei;Gan Xiaoyan;Nie Fengjie;Chen Yuchao;Shi Lei;Zhang Haiwen;Song Yuxia(Ningxia Key Laboratory of Agricultural Biotechnology, Yinchuan, 750002;Agricultural Biotechnology Research Center, Ningxia Academy of Agriculture and Forestry, Yinchuan, 750002;College of Life Sciences, Ningxia University, Yinchuan, 750003)

机构地区：[1]宁夏农业生物技术重点实验室,银川750002 [2]宁夏农林科学院农业生物技术研究中心,银川750002 [3]宁夏大学生命科学学院,银川750003

出　　处：《分子植物育种》2019年第7期2327-2332,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31560053);宁夏农业育种专项(2014NYYZ01);宁夏自然科学基金(NZ16123;NZ16122;NZ17124);宁夏农林科学院科技创新先导资金项目(NKYJ-17-19;NKYJ-17-07;NKYJ-17-21;NKYJ-17-03;NKYJ-16-12;DWHZC-2017002;NKYG-15-06);一二三产业融合发展科技创新示范项目(YES-16-0102)共同资助

摘　　要：采用分子生物学、形态学及生理检测等方法对转化HaBADH马铃薯无性一代2个株系目的基因整合情况,耐盐性鉴定及农艺性状进行了检测与分析。经PCR、RT-PCR及Southern blot分析,验证HaBADH基因稳定存在于转基因无性一代株系基因组中。当120 mmol/L NaCl持续胁迫20 d后,转基因无性一代株系表现出较强的抗盐能力,B10及B22株高平均增幅均达到15 cm,冠径平均增幅均达到12 cm,对照株高和冠径平均增幅分别达到5 cm和7 cm,平均单株结薯重量分别较对照重9 g和2 g,平均单株结薯数量较对照高0.53个和12.28个,Pro含量显著上升,MDA含量显著下降,进一步表明了转入HaBADH基因可稳定提高马铃薯的耐盐性。The molecular integration, salt tolerance identification and agronomic traits of two lines of HaBA DH potato asexual generation were detected and analyzed by approaches of molecular biology, morphology and physiological detection. By PCR, RT-PCR and Southern blot analysis, it was verified that the HaBA DH gene was stably present in the genome of the transgenic asexual generation line. When 120 mmol/L NaCl was continuously treated for 20 days, the transgenic asexual generation showed strong salt tolerance. The average increase of B10 and B22 plants reached 15 cm, and the average increase of crown diameter reached 12 cm. The average increase of crown diameter reached 5 cm and 7 cm respectively. The average weight of single potato was 9 g and 2 g, respectively. The average number of single potato was 0.53 and 12.28 higher than that of the control. The Pro content increased significantly, and the MDA content was significant. The decline further indicates that the transfer of the HaBADH gene can stably increase the salt tolerance of the potato.

关 键 词：转基因马铃薯 盐胁迫 HaBaDH 无性一代 

分 类 号：S532[农业科学—作物学]