嗜热S-腺苷甲硫氨酸合成酶的固定化及S-腺苷甲硫氨酸的制备  

Immobilization of Thermophilic S-Adenosylmethionine Synthetase and Preparation of S-Adenosylmethionine

作　　者：化浩举 吴勇 黄宗庆 张喜全 冯军 HUA Haoju;WU Yong;HUANG Zongqing;ZHANG Xiquan;FENG Jun(Shanghai Duomirui Bio-Technology Co., Ltd., Shanghai 201203;Chia-tai Tianqing Pharmaceutical Co., Ltd., Nanjing 210000)

机构地区：[1]上海多米瑞生物技术有限公司,上海201203 [2]正大天晴药业集团股份有限公司,江苏南京210000

出　　处：《中国医药工业杂志》2019年第4期406-410,共5页Chinese Journal of Pharmaceuticals

摘　　要：本研究以固定化酶比活力、酶活力回收率和反应稳定性为指标,比较了氨基树脂和环氧树脂固定S-腺苷甲硫氨酸合成酶的差别。结果显示氨基树脂固定化效果好,固定化酶比活力为(27.91±1.33)U/g,固定化酶活力回收率为(25.78±1.23)%,连续反应稳定性好。优化后固定化酶的反应条件如下:上样体积为20 BV,流速为3 BV/h,盐类型为硫酸钾与硫酸镁,产物S-腺苷甲硫氨酸的摩尔转化率达(61.78±1.91)%。固定化酶反应液经DK-1阳离子交换色谱柱纯化后得到纯度大于98%的S-腺苷甲硫氨酸(SAM),收率为(71.50±1.61)%。The specific activities of immobilized enzyme, recoveries of enzyme activity and stabilities of continuous reaction were compared between amino resin and epoxy resin in the immobilization of S-adenosylmethionine synthetase. The results showed that amino resin was an effective immobilized matrix for 5-adenosylmethionine synthetase with good stability of continuous reaction. The specific activity of immobilized enzyme and recovery rate of enzyme activity were (27.91±1.33) U/g and (25.78±1.23)%. The optimal reaction conditions of immobilized enzyme were listed as follows: the sample volume was 20 BV with the flow rate of 3 BV/h. The salt types were potassium sulfate and magnesium sulfate. The molar conversion rate of S-adenosylmethionine was up to (61.78±1.91)%. The immobilized enzyme reaction solution was purified by DK-1 cation exchange chromatographic column. S-Adenosylmethionine with the purity more than 98% was obtained and the yield was (71.50±1.61)%.

关 键 词：S-腺苷甲硫氨酸合成酶 S-腺苷甲硫氨酸 固定化 纯化 

分 类 号：TQ426.97[化学工程]