缝隙连接蛋白40磷酸化介导自发性高血压大鼠脑基底动脉血管舒张功能  

Phosphorylation of Connexin 40 mediated vasodilative function in basilar artery of rats with spontaneously hypertension

作　　者：赵小玲 王家宁[2] 何琼 ZHAO Xiao-ling;WANG Jia-ning;HE Qiong(Parmacy department,Yichang youfu hospital,Yichang 443000,Hubei,China)

机构地区：[1]宜昌市优抚医院药剂科,湖北宜昌443000 [2]湖北医药学院临床医学研究所,湖北十堰442000 [3]湖北医药学院附属人民医院心脏中心,湖北十堰442000

出　　处：《广东医学》2019年第7期937-940,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81270221)

摘　　要：目的研究自发性高血压大鼠(SHR)脑基底动脉缝隙连接蛋白40(Connexin 40, Cx40)磷酸化与血管舒张功能的变化关系。方法应用大鼠尾动脉无创血压测量技术测量Wistar大鼠和SHR尾动脉血压;压力机动图技术用于测量脑基底动脉直径的变化;免疫组织化学技术用于研究脑基底动脉平滑肌细胞Cx40的分布;Western blot技术用于研究SHR脑基底动脉平滑肌细胞Cx40的表达变化。结果相比Wistar大鼠,SHR收缩压升高(P<0.01,n=6);18β-GA抑制SNP介导的SHR脑基底动脉舒张作用减低(P<0.01,n=6);SHR脑基底动脉Cx40分布密度降低(P<0.01,n=6);SHR脑基底动脉Cx40表达下调,磷酸化Cx40表达上调(P<0.05,n=6)。结论 Cx40磷酸化,导致SHR脑基底动脉舒张功能降低,SHR收缩压上升。Objective To investigated the correlation between phosphorylation of Cx40 and the vasodilative function in basilar artery of spontaneously hypertension rats(SHR).Methods Caudal artery sphygmomanometer technology was used to measure systolic pressure of Wistar rats and SHR.Pressure myograph system technology was used to research the change of diameter of basilar artery.Immunofluorescence technique was used to study distributed density of Cx40 in basilar artery.Western blot technique were used to study expression of Cx40 in basilar artery.Results Compared with Wistar rats,SHR systolic pressure was significantly increased(P<0.01,n=6).Compared with Wistar rats,The SNP mediated vasodilative function was significantly inhibited in basilar artery of SHR(P<0.01,n=6);immunofluorescence distributed density of Cx40 were significantly reduced(P<0.01,n=6);and expression of Cx40 was significantly down-regulated and phosphorylated Cx40 was significantly up-regulated(P<0.05,n=6).Conclusion Due to phosphorylation of Cx40 mediated vasodilative function is reduced in basilar artery,SHR systolic pressure increases.

关 键 词：高血压 基底动脉 缝隙连接 压力机动图技术 

分 类 号：R544.1[医药卫生—心血管疾病] R743[医药卫生—内科学]