透皮增强肽介导的表皮生长因子调控小鼠毛发生长的研究  

Transdermal delivery peptide promoted the regulation of mice hair growth by epidermal growth factor

作　　者：金佩佩[1] 王岩石 周馨 叶书来 汪莹[3] 万昕 JIN Peipei;WANG Yanshi;ZHOU Xin;YE Shulai;WANG Ying;WAN Xin(Department of Laboratory Medicine, The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Hefei 230036, China;Reproductive Medicine Center, The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Hefei 230036, China;The First Affiliated Hospital of USTC (Anhui Provincial Hospital),Anhui Provincial Stereotactic Neurosurgical Institute, Hefei 230036, China)

机构地区：[1]中国科学技术大学附属第一医院(安徽省立医院)检验科,安徽合肥230036 [2]中国科学技术大学附属第一医院(安徽省立医院)妇产科生殖中心,安徽合肥230036 [3]中国科学技术大学附属第一医院(安徽省立医院),安徽省脑立体定向神经外科研究所,安徽合肥230036

出　　处：《中国科学技术大学学报》2018年第11期949-954,共6页JUSTC

基　　金：国家自然科学基金青年科学基金项目(81701823,31800927);国家重点研发计划(2018YFC0831101)资助.

摘　　要：TD1是一个具有11个氨基酸的短肽(ACSSSPSKHCG),能够促进蛋白药物的透皮转运进而发挥其生物学效应.前期研究显示,利用分子生物学方法得到的TD1-hEGF(T-E)融合蛋白与普通的hEGF相比,其透过完整动物皮肤的效率可提高16倍;基于蛋白的透皮效率具有TD1浓度依赖性的特点设计合成的二代TD1-hEGF:TD1-hEGF-TD1 (T-E-T)及TD1-TD1-hEGF (2T-E),具有更强的透皮效率.为进一步检测TD1介导的透皮hEGF的生物学效应,我们进行了小鼠毛发生长实验.研究发现T-E,T-E-T和2T-E这三种融合蛋白均可不同程度地诱导小鼠毛囊由休止期转为生长期,调控小鼠毛发的生长.以上结果为透皮增强肽在医学及美容等领域的应用提供了理论基础.TD1 was known as a peptide chaperon with 11 amino acids (ACSSSPSKHCG) that can facilitate transdermal delivery of proteins. Previous studies showed the maximum transdermal efficiency of fusion protein TD1-hEGF(T-E) was 16 folds higher than that of native hEGF. The second-generation of TD1-hEGF fused protein: TD1-hEGF-TD1(T-E-T) and TD1-TD1-hEGF (2T-E), designed based on the fact that transdermal efficiency was concentration-dependent of TD1, possessed considerable higher transdermal abilities than TD1-hEGF. To further detect the bioactivity of transdermal hEGF mediated by TD1, a hair growth experiments in mice was conducted. It was found that all the three fused proteins above can induce the transition of hair follicles from the telogen phase to the anagen phase of the hair cycle. These findings provide convincing evidences for improved practicability of the transdermal delivery peptide TD1 in cosmetic.

关 键 词：透皮给药 表皮生长因子 毛发生长周期 

分 类 号：R319[医药卫生—基础医学]