武陵山区稻瘟病菌生理小种分子鉴定体系的建立  

Establishment of Molecular Identification System of Magnaporthe oryzae in the Wuling Mountain Area

作　　者：刘松青 郑洁 王春台 徐鑫 LIU Song-qing;ZHENG Jie;WANG Chun-tai(Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China,Key Lab for Biotechnology of State Ethnic Affairs Commission,College of Life Science,South-Central University for Nationalities,Wuhan,Hubei 430074)

机构地区：[1]中南民族大学生命科学学院武陵山区特色资源植物种质保护与利用湖北省重点实验室生物技术国家民委重点实验室,湖北武汉430074

出　　处：《安徽农业科学》2019年第9期144-150,167,共8页Journal of Anhui Agricultural Sciences

基　　金：湖北省自然科学基金资助项目(2018CFB476);中央高校专项基金资助项目(CZD19001)

摘　　要：[目的]建立武陵山区稻瘟病菌生理小种分子鉴定体系。[方法]选用RAPD、REMAP、Rep-PCR 3种分子标记和无毒基因检测,对武陵山区稻瘟病菌生理小种文库中2013—2015年分离的220个菌株进行指纹图谱的构建和聚类分析。[结果]共统计26对引物扩增得到的3年样本中稳定出现的97条具有多态性的条带。聚类分析结果显示,在0.75的相似性水平上可将220个菌株分为12个遗传谱系,谱系10为优势谱系,不同年份的稻瘟病菌群体遗传结构存在一定差异,但并未发生明显演化。[结论]建立了新的武陵山区稻瘟病菌生理小种分子鉴定体系,为该地区抗稻瘟病菌品种的选育和布局提供参考。[Objective]To establish molecular identification system of Magnaporthe oryzae in the Wuling Mountain Area of China.[Method]Marker system including RAPD,REMAP,Rep-PCR and Avr genes was developed for fingerprint mapping and cluster analysis of 220 M.oryzae isolates collected from the Wuling Mountain Area during 2013-2015.[Result]A total of 97 polymorphic bands amplified by 26 pairs of primers were counted.The cluster analysis data showed that 220 strains could be divided into 12 genetic lineages at the 75% similarity level,and the lineage 10 was the dominant lineage.There were some differences in the genetic structure of M.oryzae populations in different years,but no significant evolution occurred.[Conclusion]The study established a new molecular identification system for M.oryzae in the Wuling Mountain Area, and provided a reference for the breeding and distribution of the M.oryzae resistant varieties in this area.

关 键 词：稻瘟病菌 遗传谱系 鉴定体系 武陵山区 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]