The barren stalk2 Gene Is Required for Axillary Meristem Development in Maize  

作　　者：Hong Yao Andrea Skirpan Brian Wardell Michaela S.Matthes Norman B.Best Tyler McCubbin Amanda Durbak Taylor Smith Simon Malcomber Paula McSteen 

机构地区：[1]Division of Biological Sciences,Interdisciplinary Plant Group,Bond Life Sciences Center,University of Missouri,Columbia,MO65211,USA [2]Department of Biology,Penn State University,University Park,PA16802,USA [3]Department of Biological Sciences,California State University,LongBeach,CA90840,USA [4]Division of Biological Sciences,Interdisciplinary Plant Group,Columbia,MO65211,USA [5]Present address:National Science Foundation,2415 Eisenhower Avenue,Alexandria,VA22314,USA

出　　处：《Molecular Plant》2019年第3期374-389,共16页分子植物（英文版）

基　　金：the National Science Foundation Plant Genome Research Program IOS-1114484/0820729 to P.M.and S.M.and NSF PGRP IOS-1546873 to P.M.

摘　　要：The diversity of plant architecture is determined by axillary meristems (AMs). AMs are produced from small groups of stem cells in the axils of leaf primordia and generate vegetative branches and reproductive inflorescences . Previous studies identified genes critical for AM development that function in auxin biosynthesis, transport, and signaling. barren stalkl (ba1), a basic helix-loop-helix transcription factor, acts downstream of auxin to control AM formation. Here, we report the cloning and characterization of barren stalk2 (ba2), a mutant that fails to produce ears and has fewer branches and spikelets in the tassel, indicating that ba2 functions in reproductive AM development. Furthermore, the ba2 mutation suppresses tiller growth in the teosinte branchedl mutant, indicating that ba2 also plays an essential role in vegetative AM development. The ba2 gene encodes a protein that co-localizes and heterodimerizes with BA1 in the nucleus . Characterization of the genetic interaction between ba2 and ba1 demonstrates that ba1 shows a gene dosage effect in ba2 mutants, providing further evidence that BA1 and BA2 act together in the same pathway. Characterization of the molecular and genetic interaction between ba2 and additional genes required for the regulation of ba1 further supports this finding. The ba1 and ba2 genes are orthologs of rice genes, LAX PANICLE1 (LAX1) and LAX2, respectively, hence providing insights into pathways controlling AMs development in grasses.

关 键 词：MAIZE AXILLARY MERISTEM inflorescenee AUXIN 

分 类 号：Q[生物学]