饲粮不同非纤维性碳水化合物/中性洗涤纤维对细毛羊氮代谢、瘤胃内环境及挥发性脂肪酸吸收的影响  

Effects of Dietary Different Non-Fiber Carbohydrate/Neutral Detergent Fiber on Nitrogen Metabolism,Rumen Environment and Volatile Fatty Acid Absorption of Fine Wool Sheep

作　　者：艳城[1] 金鹿[1] 孙海洲[1] 桑丹[1] 李胜利[1] 张春华[1] 张崇志[1] 任晓萍[1] 珊丹[1] 凌树礼[1] YAN Cheng;JIN Lu;SUN Haizhou;SANG Dan;LI Shengli;ZHANG Chunhua;ZHANG Chongzhi;REN Xiaoping;SHAN Dan;LING Shuli(Animal Nutrition and Feed Research Institution of Inner Mongolia Academy of Agriculture and Animal Husbandry Academy,Hohhot 010031,China)

机构地区：[1]内蒙古农牧业科学院动物营养与饲料研究所,呼和浩特010031

出　　处：《动物营养学报》2019年第4期1685-1694,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家绒毛用羊产业技术体系项目(CARS-40-12);内蒙古农牧业科技创新基金项目(2018CXJJM04)

摘　　要：本试验旨在研究饲粮不同非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)对细毛羊氮代谢、瘤胃内环境及挥发性脂肪酸(VFA)吸收的影响。选用9只装有永久性瘤胃瘘管的内蒙古鄂尔多斯细毛羊半同胞羯羊,按体重随机分为3组,1组、2组和3组饲粮NFC/NDF分别为0.35、0.66和1.14,粗蛋白质含量均为13.50%。结果表明:1)饲粮NFC/NDF对摄入氮、粪氮、尿氮和沉积氮无显著影响(P>0.05),1组尿中尿素氮含量显著高于2组(P<0.05),极显著高于3组(P<0.01),并且2组显著高于3组(P<0.05)。2)采食1.5和3.0 h以后,1组氨态氮(NH_3-N)含量显著高于3组(P<0.05),采食4.5 h后,各组间NH_3-N含量无显著差异(P> 0.05)。pH均在6.0～6.8,1组pH显著高于3组(P <0. 05)。3)采食1. 5 h后,1组乙酸含量显著高于2组(P <0.05),极显著高于3组(P<0.01)。采食1.5、3.5和4.0 h后,1组和2组丙酸含量显著低于3组(P<0.05)。1组采食前及采食后丁酸含量均显著高于3组(P<0.05)。4) 2组和3组Na^+/H^+交换蛋白3(NHE3)和下调式腺瘤载体(DRA)基因表达量显著高于1组(P<0.05)。1组单羧酸转运蛋白4(MCT4)基因表达量显著高于2组和3组(P <0. 05)。而2组Na^+/H^+交换蛋白1(NHE1)和钠/钾ATP酶泵(Na^+/K^+ATPase)基因表达量显著高于1组和3组(P <0. 05)。综上所述,饲粮NFC/NDF为0.66时,对细毛羊瘤胃内环境和VFA吸收相关基因表达量有显著影响。This experiment was conducted to investigate the effects of different non-fiber carbohydrate/neutral detergent fiber (NFC/NDF) on the nitrogen metabolism,rumen environment and volatile fatty acid (VFA) absorption. Nine Inner Mongolia Ordos of sheep with permanent rumen fistula were randomly divided into three groups on the basis of body weight. The NFC/NDF in diets of groups 1,2,3 were 0.35,0.66 and 1.14,respectively ,and the crude protein contents were all 13.50%. The results showed as follows: 1) the NFC/NDF had no significant differences in nitrogen intake,fecal nitrogen and nitrogen deposition ( P ﹥0.05),but the urea nitrogen content in urine in group 1 was significantly higher than that in group 2 ( P < 0.05 ),and significantly higher than that in group 3 ( P <0.01),and group 2 was significantly higher than group 3 ( P <0.05). 2) After feeding 1.5 and 3.0 h,NH 3-N content in group 1 was significantly higher than that in group 3 ( P < 0.05 ),and after feeding 4.5 h,there was no significant difference among groups ( P ﹥0.05). The pH was 6.0 to 6.8,and pH in group 1 was significantly higher than that in group 3 ( P <0.05). 3) After feeding 1.5 h,the acetic acid content in group 1 was significantly higher than that in group 2 ( P <0.05),and significantly higher than that in group 3 ( P <0.01). After feeding 1.5,3.5 and 4.0 h,the propionic acid content in groups 1 and 2 was significantly lower than that in group 3 ( P <0.05). Before feeding and after feeding,the butyric acid content in group 1 was significantly higher than that in group 3 ( P <0.05). 4) The expression levels of Na^/H + exchange protein 3 ( NHE3 )and down-regulated adenoma vector ( DRA )genes in groups 2 and 3 were significantly higher than those in group 1 ( P <0.05). The expression level of monocarboxylate transporter 4 ( MCT4 )gene in group 1 was significantly higher than that in groups 2 and 3 ( P <0.05). The expression levels of Na^/H + exchange protein 1 ( NHE1 ) and Na^/K^ ATPase pump ( Na^/ K^ ATPase ) in group 2 were significantly hig

关 键 词：细毛羊 氮代谢 瘤胃内环境 VFA吸收 

分 类 号：S826[农业科学—畜牧学]