烟草中稳定遗传转化的新技术  

New technology of stable transformation in tobacco

作　　者：杨丽萍[1,2] 王悦 徐亚男 YANG Li-ping;WANG Yue;XU Ya-nan(Key Laboratory of Jilin Province for Plant Resources Science and Green Production,Jilin Normal University,Siping 136000,China;The School of Life Science,Jilin Normal University,Siping 136000,China)

机构地区：[1]吉林师范大学吉林省植物资源科学与绿色生产重点实验室,吉林四平136000 [2]吉林师范大学生命科学学院,吉林四平136000

出　　处：《吉林师范大学学报（自然科学版）》2019年第2期106-109,共4页Journal of Jilin Normal University:Natural Science Edition

基　　金：国家青年科学基金项目(31301043);吉林省教育厅项目(JJKH20191013KJ)

摘　　要：农杆菌介导的植物稳定遗传转化技术已经在植物基因工程领域得到广泛应用.本文介绍了一种由农杆菌介导的新型植物遗传转化技术—发芽种子真空侵染法.应用此方法将表达载体p BI121-HC-Pro转化到烟草植株中,并优化了农杆菌的浓度及真空侵染的条件.半定量RT-PCR(Reverse transcription-PCR)方法检测结果表明这种转化方法可以使HC-Pro在烟草中表达,具有广泛的应用前景.Agrobacterium-mediated plant stable genetic transformation technology has been widely used in plant genetic engineering.In this paper,a new technology of plant genetic transformation mediated by Agrobacterium was introduced.The vector pBI 121 -HC-Pro into tobacco plants was transformed by using vacuum infiltration of germinated seeds,and optimized the concentration of Agrobacterium and the condition of vacuum infiltration.The results of semi- quantitative RT-PCR showed that HC-Pro gene could be stably expressed in tobacco plants by the technology.The new technology has broad application prospects.

关 键 词：新型遗传转化技术 发芽种子真空侵染法 半定量RT-PCR HC-PRO 

分 类 号：Q94[生物学—植物学]