转紫花苜蓿MsLEA2基因提高拟南芥耐铝毒性研究  

Enhancement of Aluminum Toxicity Tolerance in Arabidopsis by Transgenic Alfalfa MsLEA2

作　　者：刘星辰 吕爱敏[1] 邢强[2] 安渊[1] 周鹏[1] LIU Xing-chen;LV Ai-min;XING Qiang;AN Yuan;ZHOU Peng(College of Agriculture&Biology, Shanghai Jiaotong University, Shanghai 200240, China;Shanghai Chenshan Botanical Garden, Shanghai 201602, China)

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海辰山植物园,上海201602

出　　处：《中国草地学报》2019年第2期30-35,共6页Chinese Journal of Grassland

基　　金：国家"十三五"重大专项-科技基础资源调查专项(2017FY100600);中国南方草地牧草资源调查子课题"上海及浙江地区草地牧草资源调查";国家自然基金面上项目(31272198和31572451);上海市绿化和市容管理局科研项目(G162411)

摘　　要：MsLEA2基因是从紫花苜蓿中克隆到的胚胎晚期富集蛋白基因,属于LEA_2家族。以转基因拟南芥T_3代植株的3个株系为材料,从表型、生理和分子生物学三个方面研究铝胁迫下转MsLEA2基因拟南芥的耐铝毒性能。结果表明:铝胁迫下转基因株系的脯氨酸含量高于对照(野生型),丙二醛含量和电导率则低于对照且差异显著(P<0.05),CAT、POD和SOD活性显著高于对照。初步证明转MsLEA2基因拟南芥的耐铝毒性能明显高于对照,紫花苜蓿的MsLEA2基因具有提高植物耐铝毒胁迫的能力。The MsLEA2 gene is an late embryogenesis abundant (LEA) protein gene cloned from alfalfa and belongs to the LEA_2 family.In order to identify the resistant aluminum toxicity of the MsLEA2 gene, three strains of transgenic Arabidopsis T 3 plants were used as materials to study the resistance of transgenic Arabidopsis thaliana under aluminum stress from phenotype, physiology and molecular biology.The results showed that the proline content of the transgenic lines was higher than that of the control (wild-type) under the aluminum stress, while the content of malondialdehyde and conductivity was lower than that of the control plants, and the difference was significant (P<0.05);the CAT, POD and SOD enzyme activities of the transgenic lines were significantly higher than the control.These results preliminarily proved that the aluminum tolerance of MsLEA2 transgenic Arabidopsis thaliana was significantly higher than that of the control.The MsLEA2 gene of alfalfa had the ability to increase the tolerance to aluminum toxicity of plants.

关 键 词：植物胚胎晚期富集蛋白 铝毒 紫花苜蓿 氧化系统 

分 类 号：S541.1[农业科学—作物学]