血浆20种氨基酸水平与糖尿病风险的关联研究  

The association between plasma levels of 20 amino acids and risk of diabetes

作　　者：王凤华 刘静 邓秋菊 齐玥 王淼 王瑛[2] 孙佳艺 刘军 李岩 赵冬 Wang Fenghua;Liu Jing;Deng Qiuju;Qi Yue;Wang Miao;Wang Ying;Sun Jiayi;Liu Jun;Li Yan;Zhao Dong(Department of Epidemiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China;Research Institute of Information Technology, Tsinghua University, Beijing 100084, China)

机构地区：[1]首都医科大学附属北京安贞医院,北京市心肺血管疾病研究所流行病研究室,100029 [2]清华大学信息技术研究院,100084

出　　处：《中华内科杂志》2019年第4期270-277,共8页Chinese Journal of Internal Medicine

基　　金：国家重点基础研究发展计划项目(2012CB517806);国家科技支撑计划(2006BAI01A01,2011BA109B01).

摘　　要：目的探讨我国中老年人群血浆中20种基本氨基酸水平与糖尿病风险的关系。方法研究人群来自"中国多省市心血管疾病危险因素队列研究(CMCS)"中的首钢社区队列。2007年和2012年分别对该队列人群进行糖尿病及其他危险因素调查和血标本采集,采用液相色谱-串联质谱法对475例的2007年血样进行了多种氨基酸水平的检测。采用多因素logistic回归分析血浆氨基酸水平与糖尿病风险的关系。结果入选人群年龄为(58.7±6.3)岁,基线时血糖水平(5.68±1.34)mmol/L。横断面分析显示,在调整年龄、性别、体重指数、收缩压、血脂异常后,最高三分位水平的缬氨酸、亮氨酸、异亮氨酸、半胱氨酸与糖尿病患病的OR(95%CI)分别为3.61(1.48~8.80)、3.27(1.34~7.99)、2.46(1.04~5.84)和2.09(1.02~4.27)。前瞻性研究结果显示,基线支链氨基酸(总浓度)、苯丙氨酸、酪氨酸水平位于最高三分位的人群5年新发为糖尿病的风险分别是位于最低分位人群的3.69倍、3.61倍和4.14倍,而基线甘氨酸水平位于最高分位的人群5年新发为糖尿病的风险比最低分位的人群降低了76%(OR 0.24,95%CI 0.06~0.91)。结论血浆支链氨基酸、半胱氨酸水平升高与糖尿病的现患率的增加显著相关。支链氨基酸、芳香族氨基酸(苯丙氨酸、酪氨酸)水平较高的研究对象5年新发糖尿病的风险明显较高,而甘氨酸水平较高的研究对象5年新发糖尿病的风险明显较低。Objective To investigate the association between the plasma levels of 20 amino acids and the risk of diabetes in middle-aged and elderly population. Methods This study was a part of the Chinese multi-provincial cohort study conducted in communities of Shougang. In 2007 and 2012, the population was investigated for diabetes and other risk factors. Blood samples collected from 475 people were tested for various amino acid levels by liquid chromatography-tandem mass spectrometry. A multivariate logistic regression analysis was used to analyze the association between plasma amino acid levels and diabetes risk. Results The age of the selected population at baseline was (58.7±6.3) years, and the blood glucose level at baseline was (5.68±1.34) mmol/L. Among them, 56 (11.79%) subjects were diabetes. Multivariate logistic regression analyses showed that after adjusting for age, gender, body mass index, systolic blood pressure and dyslipidemia, individuals with plasma branched-chain amino acid (valine, leucine and isoleucine) and cysteine in the highest tertile levels were at high risk of diabetes with the ORs of 3.61 (95% CI 1.48-8.80), 3.27 (95% CI 1.34-7.99), 2.46 (95% CI 1.04-5.84) and 2.09 (95% CI 1.02-4.27), respectively. After 5 years′ followed up, 5.73%(24/419) subjects developed diabetes. Compared with those in the lowest tertile, individuals with plasma branched-chain amino acid (total concentration), phenylalanine, and tyrosine levels at baseline in the highest tertile had 3.69 times, 3.61 times and 4.14 times higher risk to develop new diabetes, respectively. In contrast, individuals with plasma glycine level in the highest tertile had only 76%(OR 0.24, 95% CI 0.06-0.91) risk for the development of diabetes compared with those with plasma glycine level in the lowest tertile. Conclusions The increase in plasma branched-chain amino acid and cysteine levels is significantly associated with an increase in incident diabetes. Subjects with higher levels of branched-chain amino acids and aromatic amino acids (phen

关 键 词：氨基酸 糖尿病 血糖 

分 类 号：R587.1[医药卫生—内分泌]