基于PCA及CA的唐古特大黄蒽醌类、二蒽酮类及鞣质类含量研究  

Research on contents of anthraquinones,dianthrones and tannins in Rheum tanguticum on PCA and CA

作　　者：黄凤[1] 尹显梅 唐国琳 连艳[1] 刘晓芬[1] 徐鑫梅 蒋桂华[1] HUANG Feng;YIN Xian-mei;TANG Guo-lin;LIAN Yan;LIU Xiao-fen;XU Xin-mei;JIANG Gui-hua(State Key Laboratory Culture Base for Systematic Study,Exploration and Utilization of Traditional Chinese Medicine Resource,Standard Key Laboratory of Traditional Chinese Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中国中药杂志》2019年第5期920-926,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(国家基础科学人才培养基金;J1310034-07);四川省科技厅应用基础计划课题(2016JY0076;2016JY019);四川省科技厅扶贫项目(2018NFP0112)

摘　　要：大黄药材中蒽醌类、二蒽酮类及鞣质类是其主要有效成分。该文以唐古特大黄主产地药材为研究对象,采用HPLC测定游离和结合蒽醌类、二蒽酮类及鞣质类成分的含量,用多重比较、主成分分析PCA及对应分析CA探究3类成分积累规律。结果显示,不同产地、不同生长方式(野生和家种)的大黄化学成分含量差异达到显著性水平(P<0. 05)。产于白玉县、小金县及若尔盖县的分别在儿茶素、大黄酸及番泻苷A的积累上具有明显的优势;唐古特大黄野生品与栽培品主成分构成不同,野生条件下,有利于结合型蒽醌组分的积累;规范化种植大黄的3类活性成分积累量优于散户零星种植。因此,唐古特大黄栽培基地选择应充分考虑产地对大黄化学成分积累的影响。规范化种植方式较优,但即使是在此条件下,唐古特大黄栽培品也不及野生品。Anthraquinones,dianthrones and tannins are the main active ingredients of Rheum tanguticum. In this study the three components were determined by HPLC,and the results were analyzed by multiple comparisons,principal components analysis(PCA)and correspondence analysis(CA). The results showed that the contents of components in different growing areas and types(wild and cultivated) reached a significant level(P<0. 05). Baiyu county,Xiaojin county and Ruoergai county had obvious advantages in the accumulation of catechin hydrate,rhien and sensenoside A respectively. The principal component was different in two growing type and the wild environment was conducive to combined anthraquinones accumulation. For active components,normalized planting was better than retail cultivating. Therefore,the effect on the accumulation of chemical components in Rh. tangusticum,should be taken into full account in the selection of the cultural base of Rh. tanguticum. The standardized cultivating is superior to retail cultivating in terms of the accumulation of active ingredients,and standardized planting is inferior to the wild.

关 键 词：唐古特大黄 蒽醌 二蒽酮 鞣质 

分 类 号：R284.1[医药卫生—中药学]